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CoraLite® Plus 488-conjugated RAD50 Polyclonal antibody

RAD50 Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-29390

Print datasheet

Synonyms

DNA repair protein RAD50, hRad50, RAD50, RAD50 2, RAD50 homolog (S. cerevisiae)

验证数据展示

  • FC experiment of HeLa using CL488-29390

    FC experiment of HeLa using CL488-29390

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RAD50 (CL488-29390) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of U2OS using CL488-29390

    IF Staining of U2OS using CL488-29390

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 RAD50 antibody (CL488-29390) at dilution of 1:200.

经过测试的应用

Positive IF detected inU2OS cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 29390-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-29390 targets RAD50 in IF, FC (Intra) applications and shows reactivity with Human, rat, mouse samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, rat, mouse
Immunogen RAD50 fusion protein Ag29949 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAD50 homolog (S. cerevisiae)
Synonyms DNA repair protein RAD50, hRad50, RAD50, RAD50 2, RAD50 homolog (S. cerevisiae)
Calculated Molecular Weight 154 kDa
Observed Molecular Weight154 kDa
GenBank Accession NumberNM_005732
Gene Symbol RAD50
Gene ID (NCBI) 10111
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92878
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RAD50 antibody CL488-29390Download protocol
FC protocol for CL Plus 488 RAD50 antibody CL488-29390Download protocol
Standard Protocols
Click here to view our Standard Protocols