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验证数据展示

  • FC experiment of HeLa using CL488-67988

    FC experiment of HeLa using CL488-67988

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RAD23B (CL488-67988, Clone:2E6G9) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL488-67988

    IF Staining of HeLa using CL488-67988

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RAD23B antibody (CL488-67988, Clone: 2E6G9 ) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated RAD23B Monoclonal antibody

RAD23B Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67988

产品说明书

CloneNo. 2E6G9

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

IF/ICC, FC (Intra)

别名

XP-C repair-complementing complex 58 kDa protein, p58, HR23B, HHR23B, 2E6G9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAD23B Ready-To-Use IHC Kit. RAD23B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67988-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67988 targets RAD23B in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原 RAD23B fusion protein Ag28658 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 RAD23 homolog B (S. cerevisiae)
别名 XP-C repair-complementing complex 58 kDa protein, p58, HR23B, HHR23B, 2E6G9
计算分子量 409 aa, 43 kDa
观测分子量58 kDa
GenBank蛋白编号BC020973
基因名称 RAD23B
Gene ID (NCBI) 5887
RRIDAB_2923850
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP54727
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAD23B, also named as p58, hHR23B and HR23B, belongs to the RAD23 family. It plays a central role both in proteosomal degradation of misfolded proteins and DNA repair. RAD23B is a central component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Involved in DNA excision repair by stabilizing XPC protein. It may play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage-processing enzymes. RAD23B has two subunit: Heterodimer of a 125 kDa subunit (p125) and of a 58 kDa subunit (p58).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RAD23B antibody CL488-67988Download protocol
FC protocol for CL Plus 488 RAD23B antibody CL488-67988Download protocol
Standard Protocols
Click here to view our Standard Protocols