• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 488-conjugated RAD23B Monoclonal antibody

RAD23B Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E6G9

Cat No : CL488-67988

Print datasheet

Synonyms

HHR23B, HR23B, P58, RAD23B

验证数据展示

  • FC experiment of HeLa using CL488-67988

    FC experiment of HeLa using CL488-67988

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RAD23B (CL488-67988, Clone:2E6G9) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL488-67988

    IF Staining of HeLa using CL488-67988

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RAD23B antibody (CL488-67988, Clone: 2E6G9 ) at dilution of 1:200, CL594-Phalloidin (red).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAD23B Ready-To-Use IHC Kit. RAD23B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67988-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67988 targets RAD23B in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen RAD23B fusion protein Ag28658 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name RAD23 homolog B (S. cerevisiae)
Synonyms HHR23B, HR23B, P58, RAD23B
Calculated Molecular Weight 409 aa, 43 kDa
Observed Molecular Weight58 kDa
GenBank Accession NumberBC020973
Gene Symbol RAD23B
Gene ID (NCBI) 5887
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54727
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAD23B, also named as p58, hHR23B and HR23B, belongs to the RAD23 family. It plays a central role both in proteosomal degradation of misfolded proteins and DNA repair. RAD23B is a central component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Involved in DNA excision repair by stabilizing XPC protein. It may play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage-processing enzymes. RAD23B has two subunit: Heterodimer of a 125 kDa subunit (p125) and of a 58 kDa subunit (p58).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RAD23B antibody CL488-67988Download protocol
FC protocol for CL Plus 488 RAD23B antibody CL488-67988Download protocol
Standard Protocols
Click here to view our Standard Protocols