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验证数据展示

  • WB analysis using 84920-4-RR

    WB analysis using 84920-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84920-4-RR (RAD21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84920-4-RR

    IF Staining of HeLa using 84920-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAD21 antibody (84920-4-RR, Clone: 242172E3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of Jurkat using 84920-4-RR

    FC experiment of Jurkat using 84920-4-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug RAD21 Recombinant antibody (84920-4-RR, Clone:242172E3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of HeLa using 84920-4-RR

    WB analysis of HeLa using 84920-4-RR

    WB result of RAD21 antibody (84920-4-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAD21 transfected HeLa cells.

  • Affinity and Kinetic Characterization of 84920-4-RR

    Affinity and Kinetic Characterization of 84920-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84920-4-RR against Human RAD21 were performed. The affinity constant is 2.23 nM.

  • Featured Product
  • KD/KO验证

RAD21 Recombinant antibody

RAD21 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84920-4-RR

产品说明书

CloneNo. 242172E3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), ELISA

别名

hHR21, FLJ40596, FLJ25655, Double-strand-break repair protein rad21 homolog, 64-kDa C-terminal product

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy RAD21 Ready-To-Use IHC Kit. RAD21 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84920-4-RR targets RAD21 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RAD21 fusion protein Ag25481 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAD21 homolog (S. pombe)
别名 hHR21, FLJ40596, FLJ25655, Double-strand-break repair protein rad21 homolog, 64-kDa C-terminal product
计算分子量 631 aa, 72 kDa
观测分子量 140 kDa
GenBank蛋白编号BC050381
基因名称 RAD21
Gene ID (NCBI) 5885
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO60216
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAD21, also named as Nuclear matrix protein 1, is a 631 amino acid protein, which belongs to the rad21 family. The calculated molecular weight of RAD21 is 72 kDa, but the phosphorylated RAD21 is about 120 kDa. The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.

实验方案

Product Specific Protocols
WB protocol for RAD21 antibody 84920-4-RRDownload protocol
IF protocol for RAD21 antibody 84920-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols