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验证数据展示

  • IP experiment of K-562 using 18333-1-AP (same clone as 18333-1-PBS)

    IP experiment of K-562 using 18333-1-AP (same clone as 18333-1-PBS)

    IP result of anti-RAD18 (IP:18333-1-AP, 3ug; Detection:18333-1-AP 1:300) with K-562 cells lysate 2000ug. This data was developed using the same antibody clone with 18333-1-PBS in a different storage buffer formulation.

  • WB analysis using 18333-1-AP (same clone as 18333-1-PBS)

    WB analysis using 18333-1-AP (same clone as 18333-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18333-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 18333-1-AP (same clone as 18333-1-PBS)

    IHC staining of human ovary cancer using 18333-1-AP (same clone as 18333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18333-1-AP (RAD18 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18333-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 18333-1-AP (same clone as 18333-1-PBS)

    IF Staining of MCF-7 using 18333-1-AP (same clone as 18333-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RAD18 antibody (18333-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 18333-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RAD18 Polyclonal antibody, PBS Only

RAD18 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 18333-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

E3 ubiquitin-protein ligase RAD18, EC:2.3.2.27, hHR18, hRAD18, RING finger protein 73

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

18333-1-PBS targets RAD18 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag12944

Product name: Recombinant human RAD18 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-495 aa of BC001302

Sequence: MDSLAESRWPPGLAVMKTIDDLLRCGICFEYFNIAMIIPQCSHNYCSLCIRKFLSYKTQCPTCCVTVTEPDLKNNRILDELVKSLNFARNHLLQFALESPAKSPASSSSKNLAVKVYTPVASRQSLKQGSRLMDNFLIREMSGSTSELLIKENKSKFSPQKEASPAAKTKETRSVEEIAPDPSEAKRPEPPSTSTLKQVTKVDCPVCGVNIPESHINKHLDSCLSREEKKESLRSSVHKRKPLPKTVYNLLSDRDLKKKLKEHGLSIQGNKQQLIKRHQEFVHMYNAQCDALHPKSAAEIVQEIENIEKTRMRLEASKLNESVMVFTKDQTEKEIDEIHSKYRKKHKSEFQLLVDQARKGYKKIAGMSQKTVTITKEDESTEKLSSVCMGQEDNMTSVTNHFSQSKLDSPEELEPDREEDSSSCIDIQEVLSSSESDSCNSSSSDIIRDLLEEEEAWEASHKNDLQDTEISPRQNRRTRAAESAEIEPRNKRNRN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAD18 homolog (S. cerevisiae)
别名 E3 ubiquitin-protein ligase RAD18, EC:2.3.2.27, hHR18, hRAD18, RING finger protein 73
计算分子量 56 kDa
观测分子量 75 kDa, 85 kDa
GenBank蛋白编号BC001302
基因名称 RAD18
Gene ID (NCBI) 56852
RRIDAB_2176586
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NS91
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAD18(E3 ubiquitin-protein ligase RAD18) is lalso named as RNF73, hHR18 and belongs to the RAD18 family. It is involved in postreplication repair of UV-damaged DNA. RAD18 protein is detected in human cells as two major bands at 75 and 85 kDa by Western blot. The bands are identified as nonubiquitinated and monoubiquitinated forms of Rad18, respectively(PMID: 15509568). Monoubiquitinated RAD18 is detected mainly in the cytoplasm, whereas nonubiquitinated RAD18 is detected predominantly in the nuclei(PMID:15509568). This antibody is specific to RAD18.

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