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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68637-1-Ig (same clone as 68637-1-PBS)

    WB analysis using 68637-1-Ig (same clone as 68637-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68637-1-Ig (RAD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68637-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 68637-1-Ig (same clone as 68637-1-PBS)

    IP experiment of HeLa using 68637-1-Ig (same clone as 68637-1-PBS)

    IP result of anti-RAD1 (IP:68637-1-Ig, 4ug; Detection:68637-1-Ig 1:1000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 68637-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68637-1-Ig (same clone as 68637-1-PBS)

    FC experiment of HeLa using 68637-1-Ig (same clone as 68637-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAD1 Monoclonal antibody (68637-1-Ig, Clone:1A11E2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68637-1-PBS in a different storage buffer formulation.

RAD1 Monoclonal antibody, PBS Only

RAD1 Monoclonal Antibody for WB, FC (Intra), IP, Indirect ELISA
Cat No. 68637-1-PBS

产品说明书

CloneNo. 1A11E2

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, FC (Intra), IP, Indirect ELISA

别名

REC1, Rad1-like DNA damage checkpoint protein, HRAD1, 1A11E2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68637-1-PBS targets RAD1 in WB, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 RAD1 fusion protein Ag33257 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 RAD1 homolog (S. pombe)
别名 REC1, Rad1-like DNA damage checkpoint protein, HRAD1, 1A11E2
计算分子量 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC006837
基因名称 RAD1
Gene ID (NCBI) 5810
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO60671
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAD1 is also named as REC1,hRAD1. The human RAD1 and HUS1 proteins associate in a complex that interacts with a highly modified form of RAD9. They concluded that these 3 proteins are central components of a DNA damage-responsive protein complex in human cells.It has 3 isoforms produced by alternative splicing.