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验证数据展示

  • WB analysis using 68637-1-Ig (same clone as 68637-1-PBS)

    WB analysis using 68637-1-Ig (same clone as 68637-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68637-1-Ig (RAD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68637-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 68637-1-Ig (same clone as 68637-1-PBS)

    IP experiment of HeLa using 68637-1-Ig (same clone as 68637-1-PBS)

    IP result of anti-RAD1 (IP:68637-1-Ig, 4ug; Detection:68637-1-Ig 1:1000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 68637-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68637-1-Ig (same clone as 68637-1-PBS)

    FC experiment of HeLa using 68637-1-Ig (same clone as 68637-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAD1 Monoclonal antibody (68637-1-Ig, Clone:1A11E2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68637-1-PBS in a different storage buffer formulation.

RAD1 Monoclonal antibody, PBS Only

RAD1 Monoclonal Antibody for WB, FC (Intra), IP, Indirect ELISA
Cat No. 68637-1-PBS

产品说明书

CloneNo. 1A11E2

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, FC (Intra), IP, Indirect ELISA

别名

REC1, Rad1-like DNA damage checkpoint protein, HRAD1, 1A11E2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68637-1-PBS targets RAD1 in WB, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33257

Product name: Recombinant human RAD1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-282 aa of BC006837

Sequence: MPLLTQQIQDEDDQYSLVASLDNVRNLSTILKAIHFREHATCFATKNGIKVTVENAKCVQANAFIQAGIFQEFKVQEESVTFRINLTVLLDCLSIFGSSPMPGTLTALRMCYQGYGYPLMLFLEEGGVVTVCKINTQEPEETLDFDFCSTNVINKIILQSEGLREAFSELDMTSEVLQITMSPDKPYFRLSTFGNAGSSHLDYPKDSDLMEAFHCNQTQVNRYKISLLKPSTKALVLSCKVSIRTDNRGFLSLQYMIRNEDGQICFVEYYCCPDEEVPESES

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 RAD1 homolog (S. pombe)
别名 REC1, Rad1-like DNA damage checkpoint protein, HRAD1, 1A11E2
计算分子量 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC006837
基因名称 RAD1
Gene ID (NCBI) 5810
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO60671
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAD1 is also named as REC1,hRAD1. The human RAD1 and HUS1 proteins associate in a complex that interacts with a highly modified form of RAD9. They concluded that these 3 proteins are central components of a DNA damage-responsive protein complex in human cells.It has 3 isoforms produced by alternative splicing.

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