• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68637-1-Ig

    WB analysis using 68637-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68637-1-Ig (RAD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 68637-1-Ig

    IP experiment of HeLa using 68637-1-Ig

    IP result of anti-RAD1 (IP:68637-1-Ig, 4ug; Detection:68637-1-Ig 1:1000) with HeLa cells lysate 1320 ug.

  • FC experiment of HeLa using 68637-1-Ig

    FC experiment of HeLa using 68637-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAD1 Monoclonal antibody (68637-1-Ig, Clone:1A11E2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

RAD1 Monoclonal antibody

RAD1 Monoclonal Antibody for WB, FC (Intra), IP, ELISA
Cat No. 68637-1-Ig

产品说明书

CloneNo. 1A11E2

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, FC (Intra), IP, ELISA

别名

REC1, Rad1-like DNA damage checkpoint protein, HRAD1, 1A11E2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inMKN-45 cells, U2OS cells, HeLa cells, HEK-293 cells, Jurkat cells, MOLT-4 cells, Raji cells, Daudi cells, K-562 cells
Positive IP detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68637-1-Ig targets RAD1 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), IP, ELISA Application Description
经测试反应性 human
免疫原 RAD1 fusion protein Ag33257 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 RAD1 homolog (S. pombe)
别名 REC1, Rad1-like DNA damage checkpoint protein, HRAD1, 1A11E2
计算分子量 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC006837
基因名称 RAD1
Gene ID (NCBI) 5810
RRIDAB_3085326
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO60671
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAD1 is also named as REC1,hRAD1. The human RAD1 and HUS1 proteins associate in a complex that interacts with a highly modified form of RAD9. They concluded that these 3 proteins are central components of a DNA damage-responsive protein complex in human cells.It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for RAD1 antibody 68637-1-IgDownload protocol
IP protocol for RAD1 antibody 68637-1-IgDownload protocol
FC protocol for RAD1 antibody 68637-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols