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验证数据展示

  • WB analysis using 68637-1-Ig

    WB analysis using 68637-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68637-1-Ig (RAD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 68637-1-Ig

    IP experiment of HeLa using 68637-1-Ig

    IP result of anti-RAD1 (IP:68637-1-Ig, 4ug; Detection:68637-1-Ig 1:1000) with HeLa cells lysate 1320 ug.

  • FC experiment of HeLa using 68637-1-Ig

    FC experiment of HeLa using 68637-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAD1 Monoclonal antibody (68637-1-Ig, Clone:1A11E2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

RAD1 Monoclonal antibody

RAD1 Monoclonal Antibody for WB, FC (Intra), IP, ELISA
Cat No. 68637-1-Ig

产品说明书

CloneNo. 1A11E2

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, FC (Intra), IP, ELISA

别名

REC1, Rad1-like DNA damage checkpoint protein, HRAD1, 1A11E2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMKN-45 cells, U2OS cells, HeLa cells, HEK-293 cells, Jurkat cells, MOLT-4 cells, Raji cells, Daudi cells, K-562 cells
Positive IP detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68637-1-Ig targets RAD1 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33257

Product name: Recombinant human RAD1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-282 aa of BC006837

Sequence: MPLLTQQIQDEDDQYSLVASLDNVRNLSTILKAIHFREHATCFATKNGIKVTVENAKCVQANAFIQAGIFQEFKVQEESVTFRINLTVLLDCLSIFGSSPMPGTLTALRMCYQGYGYPLMLFLEEGGVVTVCKINTQEPEETLDFDFCSTNVINKIILQSEGLREAFSELDMTSEVLQITMSPDKPYFRLSTFGNAGSSHLDYPKDSDLMEAFHCNQTQVNRYKISLLKPSTKALVLSCKVSIRTDNRGFLSLQYMIRNEDGQICFVEYYCCPDEEVPESES

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 RAD1 homolog (S. pombe)
别名 REC1, Rad1-like DNA damage checkpoint protein, HRAD1, 1A11E2
计算分子量 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC006837
基因名称 RAD1
Gene ID (NCBI) 5810
RRIDAB_3085326
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO60671
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAD1 is also named as REC1,hRAD1. The human RAD1 and HUS1 proteins associate in a complex that interacts with a highly modified form of RAD9. They concluded that these 3 proteins are central components of a DNA damage-responsive protein complex in human cells.It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for RAD1 antibody 68637-1-IgDownload protocol
IP protocol for RAD1 antibody 68637-1-IgDownload protocol
FC protocol for RAD1 antibody 68637-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols