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验证数据展示

  • WB analysis using 88471-1-RR

    WB analysis using 88471-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88471-1-RR (RAC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 88471-1-RR

    IF Staining of HeLa using 88471-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAC1 antibody (88471-1-RR, Clone: 260819A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of rat brain using 88471-1-RR

    IHC staining of rat brain using 88471-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 88471-1-RR (RAC1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAC1 antibody included

RAC1 Recombinant monoclonal antibody

RAC1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 88471-1-RR

产品说明书

CloneNo. 260819A6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

MIG5, Cell migration-inducing gene 5 protein, EC:3.6.5.2, p21 Rac1, p21-Rac1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HUVEC cells, HL-60 cells, RAW 264.7 cells, HSC-T6 cells, mouse brain tissue
Positive IHC detected inrat brain tissue, mouse brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAC1 Ready-To-Use IHC Kit. RAC1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

88471-1-RR targets RAC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0751

Product name: Recombinant human RAC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-192 aa of BC004247

Sequence: MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLIPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
别名 MIG5, Cell migration-inducing gene 5 protein, EC:3.6.5.2, p21 Rac1, p21-Rac1
计算分子量 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC004247
基因名称 RAC1
Gene ID (NCBI) 5879
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP63000
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.

实验方案

Product Specific Protocols
IF protocol for RAC1 antibody 88471-1-RRDownload protocol
IHC protocol for RAC1 antibody 88471-1-RRDownload protocol
WB protocol for RAC1 antibody 88471-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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