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验证数据展示

  • WB analysis using 83470-3-RR (same clone as 83470-3-PBS)

    WB analysis using 83470-3-RR (same clone as 83470-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83470-3-RR (RABL2B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83470-3-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83470-3-RR (same clone as 83470-3-PBS)

    FC experiment of A549 using 83470-3-RR (same clone as 83470-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug RABL2B Recombinant antibody (83470-3-RR, Clone:240501D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83470-3-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 83470-3-RR (same clone as 83470-3-PBS)

    FC experiment of MCF-7 using 83470-3-RR (same clone as 83470-3-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RABL2B Recombinant antibody (83470-3-RR, Clone:240501D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83470-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83470-3-RR

    Affinity and Kinetic Characterization of 83470-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83470-3-RR against Human RABL2B were performed. The affinity constant is 0.256 nM.

RABL2B Recombinant monoclonal antibody, PBS Only

RABL2B Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83470-3-PBS

产品说明书

CloneNo. 240501D3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

240501D3, Rab like protein 2B, Rab-like protein 2B

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83470-3-PBS targets RABL2B in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2189

Product name: Recombinant human RABL2B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-229 aa of BC024281

Sequence: MAEDKTKPSELDQGKYDADDNVKIICLGDSAVGKSKLMERFLMDGFQPQQLSTYALTLYKHTATVDGRTILVDFWDTAGQERFQSMHASYYHKAHACIMVFDVQRKVTYRNLSTWYTELREFRPEIPCIVVANKIDADINVTQKSFNFAKKFSLPLYFVSAADGTNVVKLFNDAIRLAVSYKQNSQDFMDEIFQELENFSLEQEEEDVPDQEQSSSIETPSEEAASPHS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAB, member of RAS oncogene family-like 2B
别名 240501D3, Rab like protein 2B, Rab-like protein 2B
计算分子量 229 aa, 26 kDa
GenBank蛋白编号BC024281
基因名称 RABL2B
Gene ID (NCBI) 11158
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UNT1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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