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RABL2B Polyclonal antibody

RABL2B Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 11588-1-AP

Print datasheet

Synonyms

Rab like protein 2B, RABL2B

验证数据展示

  • WB analysis using 11588-1-AP

    WB analysis using 11588-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11588-1-AP (RABL2B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 11588-1-AP

    IHC staining of human lung cancer using 11588-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 11588-1-AP (RABL2B antibody) at dilution of 1:50 (under 10x lens).

  • IP experiment of fetal human brain using 11588-1-AP

    IP experiment of fetal human brain using 11588-1-AP

    IP result of anti-RABL2B (IP:11588-1-AP, 4ug; Detection:11588-1-AP 1:300) with fetal human brain tissue lysate 4000 ug.

经过测试的应用

Positive WB detected infetal human brain tissue, human brain tissue, human kidney tissue, human liver tissue, human heart tissue, mouse brain tissue
Positive IP detected infetal human brain tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11588-1-AP targets RABL2B in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen RABL2B fusion protein Ag2189 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB, member of RAS oncogene family-like 2B
Synonyms Rab like protein 2B, RABL2B
Calculated Molecular Weight 229 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC024281
Gene Symbol RABL2B
Gene ID (NCBI) 11158
RRIDAB_2175954
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UNT1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RABL2B antibody 11588-1-APDownload protocol
IHC protocol for RABL2B antibody 11588-1-APDownload protocol
IP protocol for RABL2B antibody 11588-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

J Cell Sci

RABL2 positively controls localization of GPCRs in mammalian primary cilia.

Authors - Izumi Dateyama
View Article