验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, HepG2 cells, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, mouse brain tissue |
| Positive IP detected in | fetal human brain tissue |
| Positive IHC detected in | mouse kidney tissue, human lung cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 1 publications below |
产品信息
11588-1-AP targets RABL2B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | RABL2B fusion protein Ag2189 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB, member of RAS oncogene family-like 2B |
| 别名 | Rab like protein 2B, Rab-like protein 2B |
| 计算分子量 | 229 aa, 26 kDa |
| 观测分子量 | 26 kDa |
| GenBank蛋白编号 | BC024281 |
| 基因名称 | RABL2B |
| Gene ID (NCBI) | 11158 |
| RRID | AB_2175954 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UNT1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Rab-like protein 2B (RABL2B) is a member of a poorly characterised clade of the RAS GTPase superfamily, which plays an essential role in male fertility, sperm intraflagellar transport and tail assembly (PMID: 28553998, PMID: 28138870). A preferential expression of RABL2B in human tissues and lymphoblastoid cell lines was detected which is most pronounced in brain and placenta (PMID: 20138207).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RABL2B antibody 11588-1-AP | Download protocol |
| IHC protocol for RABL2B antibody 11588-1-AP | Download protocol |
| IP protocol for RABL2B antibody 11588-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
