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验证数据展示

  • WB analysis of Jurkat using 66622-1-Ig (same clone as 66622-1-PBS)

    WB analysis of Jurkat using 66622-1-Ig (same clone as 66622-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66622-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66622-1-Ig (same clone as 66622-1-PBS)

    IHC staining of human lung cancer using 66622-1-Ig (same clone as 66622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66622-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RABEPK antibody included
  • IHC staining of human lung cancer using 66622-1-Ig (same clone as 66622-1-PBS)

    IHC staining of human lung cancer using 66622-1-Ig (same clone as 66622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66622-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RABEPK antibody included
  • IF Staining of A549 using 66622-1-Ig (same clone as 66622-1-PBS)

    IF Staining of A549 using 66622-1-Ig (same clone as 66622-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RABEPK/p40 antibody (66622-1-Ig, Clone: 1E11A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66622-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66622-1-Ig (same clone as 66622-1-PBS)

    IF Staining of HeLa using 66622-1-Ig (same clone as 66622-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RABEPK/p40 antibody (66622-1-Ig, Clone: 1E11A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66622-1-PBS in a different storage buffer formulation.

RABEPK/p40 Monoclonal antibody, PBS Only

RABEPK/p40 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66622-1-PBS

产品说明书

CloneNo. 1E11A3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

p40, RABEPK, RAB9P40, Rab9 effector protein with kelch motifs, 40 kDa Rab9 effector protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy RABEPK Ready-To-Use IHC Kit. RABEPK primary antibody included.

产品信息

66622-1-PBS targets RABEPK/p40 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RABEPK/p40 fusion protein Ag7796 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Rab9 effector protein with kelch motifs
别名 p40, RABEPK, RAB9P40, Rab9 effector protein with kelch motifs, 40 kDa Rab9 effector protein
计算分子量 41 kDa
观测分子量 40 kDa
GenBank蛋白编号BC065725
基因名称 RABEPK/p40
Gene ID (NCBI) 10244
RRIDAB_2881982
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ7Z6M1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network.