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验证数据展示

  • WB analysis of Jurkat using 60125-1-Ig

    WB analysis of Jurkat using 60125-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60125-1-Ig (RABEP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 60125-1-Ig

    WB analysis of HSC-T6 using 60125-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60125-1-Ig (RABEP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 60125-1-Ig

    IHC staining of human prostate cancer using 60125-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60125-1-Ig (RABEP2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 60125-1-Ig

    IHC staining of human prostate cancer using 60125-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60125-1-Ig (RABEP2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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RABEP2 Monoclonal antibody

RABEP2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60125-1-Ig

产品说明书

CloneNo. 1A4A4

宿主/亚型

Mouse / IgG1

种属反应性

human, rat, pig

应用

WB, IHC, ELISA

别名

1A4A4, FRA, Rab GTPase-binding effector protein 2, Rabaptin-5beta, RABEP 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHSC-T6 cells, Jurkat cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60125-1-Ig targets RABEP2 in WB, IHC, ELISA applications and shows reactivity with human, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, rat, pig
免疫原

CatNo: Ag6197

Product name: Recombinant human RABEP2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-299 aa of BC058900

Sequence: MAAAAPVAADDDERRRRPGAALEDSRSQEGANGEAESGELSRLRAELAGALAEMETMKAVAEVSESTKAEAVAAVQRQCQEEVASLQAILKDSISSYEAQITALKQERQQQQQDCEEKERELGRLKQLLSRAYPLDSLEKQMEKAHEDSEKLREIVLPMEKEIEELKAKLLRAEELIQEIQRRPRHAPSLHGSTELLPLSRDPSPPLEPLEELSGDGGPAAEAFAHNCDDSASISSFSLGGGVGSSSSLPQSRQGLSPEQEETASLVSTGTLVPEGIYLPPPGYQLVPDTQWEQLQTEM

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 rabaptin, RAB GTPase binding effector protein 2
别名 1A4A4, FRA, Rab GTPase-binding effector protein 2, Rabaptin-5beta, RABEP 2
计算分子量 64 kDa
观测分子量 62 kDa
GenBank蛋白编号BC058900
基因名称 RABEP2
Gene ID (NCBI) 79874
RRIDAB_2284821
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9H5N1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RABEP2, also named as RABPT5B, plays a role in membrane trafficking and in homotypic early endosome fusion. It has two isoforms with MW 59-63 kDa.

实验方案

Product Specific Protocols
IHC protocol for RABEP2 antibody 60125-1-IgDownload protocol
WB protocol for RABEP2 antibody 60125-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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