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验证数据展示

  • WB analysis using 84129-5-RR

    WB analysis using 84129-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84129-5-RR (RAB9A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of U2OS using 84129-5-RR

    FC experiment of U2OS using 84129-5-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug RAB9A Recombinant antibody (84129-5-RR, Clone:241372F8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 84129-5-RR

    Affinity and Kinetic Characterization of 84129-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84129-5-RR against Human RAB9A were performed. The affinity constant is 11.9 nM.

RAB9A Recombinant monoclonal antibody

RAB9A Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84129-5-RR

产品说明书

CloneNo. 241372F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

RAB9, 241372F8, Ras related protein Rab 9A, Ras-related protein Rab-9A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, NIH/3T3 cells
Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy RAB9A Ready-To-Use IHC Kit. RAB9A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84129-5-RR targets RAB9A in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1969

Product name: Recombinant human RAB9A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-201 aa of BC017265

Sequence: MAGKSSLFKVILLGDGGVGKSSLMNRYVTNKFDTQLFHTIGVEFLNKDLEVDGHFVTMQIWDTAGQERFRSLRTPFYRGSDCCLLTFSVDDSQSFQNLSNWKKEFIYYADVKEPESFPFVILGNKIDISERQVSTEEAQAWCRDNGDYPYFETSAKDATNVAAAFEEAVRRVLATEDRSDHLIQTDTVNLHRKPKPSSSCC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAB9A, member RAS oncogene family
别名 RAB9, 241372F8, Ras related protein Rab 9A, Ras-related protein Rab-9A
计算分子量 201 aa, 23 kDa
观测分子量 23 kDa
GenBank蛋白编号BC017265
基因名称 RAB9A
Gene ID (NCBI) 9367
RRIDAB_3671688
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP51151
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB9A belongs to the small GTPase superfamily, the members of which play an important role in regulating vesicular trafficking and reside in specific intracellular compartments. RAB9A has been localized to components of the endocytic/exocytic pathway and has been implicated in recycling of membrane receptors, such as mannose 6-phosphate receptor, from early endosomes to the trans Golgi network.

实验方案

Product Specific Protocols
WB protocol for RAB9A antibody 84129-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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