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验证数据展示

  • WB analysis using 84129-5-RR (same clone as 84129-5-PBS)

    WB analysis using 84129-5-RR (same clone as 84129-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84129-5-RR (RAB9A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84129-5-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 84129-5-RR (same clone as 84129-5-PBS)

    FC experiment of U2OS using 84129-5-RR (same clone as 84129-5-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug RAB9A Recombinant antibody (84129-5-RR, Clone:241372F8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84129-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84129-5-RR

    Affinity and Kinetic Characterization of 84129-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84129-5-RR against Human RAB9A were performed. The affinity constant is 11.9 nM.

RAB9A Recombinant monoclonal antibody, PBS Only

RAB9A Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84129-5-PBS

产品说明书

CloneNo. 241372F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

RAB9, 241372F8, Ras related protein Rab 9A, Ras-related protein Rab-9A

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RAB9A Ready-To-Use IHC Kit. RAB9A primary antibody included.

产品信息

84129-5-PBS targets RAB9A in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1969

Product name: Recombinant human RAB9A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-201 aa of BC017265

Sequence: MAGKSSLFKVILLGDGGVGKSSLMNRYVTNKFDTQLFHTIGVEFLNKDLEVDGHFVTMQIWDTAGQERFRSLRTPFYRGSDCCLLTFSVDDSQSFQNLSNWKKEFIYYADVKEPESFPFVILGNKIDISERQVSTEEAQAWCRDNGDYPYFETSAKDATNVAAAFEEAVRRVLATEDRSDHLIQTDTVNLHRKPKPSSSCC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RAB9A, member RAS oncogene family
别名 RAB9, 241372F8, Ras related protein Rab 9A, Ras-related protein Rab-9A
计算分子量 201 aa, 23 kDa
观测分子量 23 kDa
GenBank蛋白编号BC017265
基因名称 RAB9A
Gene ID (NCBI) 9367
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP51151
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RAB9A belongs to the small GTPase superfamily, the members of which play an important role in regulating vesicular trafficking and reside in specific intracellular compartments. RAB9A has been localized to components of the endocytic/exocytic pathway and has been implicated in recycling of membrane receptors, such as mannose 6-phosphate receptor, from early endosomes to the trans Golgi network.

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