RAB5A Monoclonal antibody
RAB5A Monoclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, pig
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1B6A5
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, fetal human brain tissue, pig brain tissue |
Positive IHC detected in | human liver cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells, HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IF | See 2 publications below |
产品信息
66339-1-Ig targets RAB5A in WB, IF, IHC, ELISA applications and shows reactivity with human, pig samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IF |
Tested Reactivity | human, pig |
Cited Reactivity | human |
Immunogen | RAB5A fusion protein Ag16781 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | RAB5A, member RAS oncogene family |
Synonyms | RAB5, RAB5A, Ras related protein Rab 5A |
Calculated Molecular Weight | 215 aa, 24 kDa |
Observed Molecular Weight | 24 kDa |
GenBank Accession Number | BC001267 |
Gene Symbol | RAB5A |
Gene ID (NCBI) | 5868 |
RRID | AB_2881719 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P20339 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for RAB5A antibody 66339-1-Ig | Download protocol |
IHC protocol for RAB5A antibody 66339-1-Ig | Download protocol |
IF protocol for RAB5A antibody 66339-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Bioact Mater Matrix stiffness exacerbates the proinflammatory responses of vascular smooth muscle cell through the DDR1-DNMT1 mechanotransduction axis. | ||
Clin Ther Differential Expression of Rab5 and Rab7 Small GTPase Proteins in Placental Tissues From Pregnancies Affected by Maternal Coronavirus Disease 2019. | ||
J Exp Clin Cancer Res TFEB-driven autophagy potentiates TGF-β induced migration in pancreatic cancer cells. | ||
Commun Biol Arginine GlcNAcylation of Rab small GTPases by the pathogen Salmonella Typhimurium. |