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RAB3IP/Rabin8 Polyclonal antibody

RAB3IP/Rabin8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12321-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

RAB3IP, Rabin8, Rabin 3, Rab3A-interacting protein, Rab-3A-interacting protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, human heart tissue, mouse lung tissue, human brain tissue, mouse heart tissue, rat brain tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12321-1-AP targets RAB3IP/Rabin8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, canine
免疫原 RAB3IP/Rabin8 fusion protein Ag2974 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB3A interacting protein (rabin3)
别名 RAB3IP, Rabin8, Rabin 3, Rab3A-interacting protein, Rab-3A-interacting protein
计算分子量 476 aa, 53 kDa
观测分子量 53/51/43 kDa
GenBank蛋白编号BC015548
基因名称 RAB3IP
Gene ID (NCBI) 117177
RRIDAB_2177510
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96QF0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB3IP, also hnown as Rabin8, is a deduced 476-amino acid protein containing a central coiled-coil domain, a bipartite nuclear localization signal, and a C-terminal endoplasmic reticulum retention motif. Rabin8 has several sites for N-glycosylation and phosphorylation. Rabin8 plays important roles in various cellular processes, including ciliogenesis.The GEF activity of Rabin8 towards Rab8 is essential for ciliogenesis and cyst apical membrane transport,but not for exocytosis of discoidal/fusiform-shaped vesicles. This antibody can recognize isoforms with predicted MW's of 53 kDa, 51 kDa, 43 kDa, 45 kDa, 33 kDa and 35 kDa, 29 kDa respectively.

实验方案

Product Specific Protocols
WB protocol for RAB3IP/Rabin8 antibody 12321-1-APDownload protocol
IHC protocol for RAB3IP/Rabin8 antibody 12321-1-APDownload protocol
IF protocol for RAB3IP/Rabin8 antibody 12321-1-APDownload protocol
IP protocol for RAB3IP/Rabin8 antibody 12321-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
canineWB,IF

Nat Cell Biol

A molecular network for de novo generation of the apical surface and lumen.

Authors - Bryant David M DM
  • KD Validated
ratWB, IF

Neuron

Chemical genetic identification of NDR1/2 kinase substrates AAK1 and Rabin8 Uncovers their roles in dendrite arborization and spine development.

Authors - Sila K Ultanir
mouseWB, IF

PLoS Biol

GSK3β-Dzip1-Rab8 Cascade Regulates Ciliogenesis after Mitosis.

Authors - Boyan Zhang
humanWB

J Cell Biol

Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation.

Authors - Michael C Burke
humanWB

EMBO J

Phosphoproteomic screening identifies Rab GTPases as novel downstream targets of PINK1.

Authors - Yu-Chiang Lai
humanWB

EMBO J

Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.

Authors - Tatsuo Miyamoto
  • KO Validated
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