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验证数据展示

  • WB analysis using 10788-1-AP (same clone as 10788-1-PBS)

    WB analysis using 10788-1-AP (same clone as 10788-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10788-1-AP (RAB3C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10788-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 10788-1-AP (same clone as 10788-1-PBS)

    IP experiment of mouse brain using 10788-1-AP (same clone as 10788-1-PBS)

    IP result of anti-RAB3C (IP:10788-1-AP, 4ug; Detection:10788-1-AP 1:300) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 10788-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 10788-1-AP (same clone as 10788-1-PBS)

    IHC staining of mouse brain using 10788-1-AP (same clone as 10788-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10788-1-AP (RAB3C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10788-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 10788-1-AP (same clone as 10788-1-PBS)

    IHC staining of mouse brain using 10788-1-AP (same clone as 10788-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10788-1-AP (RAB3C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10788-1-PBS in a different storage buffer formulation.

RAB3C Polyclonal antibody, PBS Only

RAB3C Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 10788-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

10788-1-PBS targets RAB3C in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1241

Product name: Recombinant human RAB3C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-227 aa of BC013033

Sequence: MRHEAPMQMASAQDARYGQKDSSDQNFDYMFKLLIIGNSSVGKTSFLFRYADDSFTSAFVSTVGIDFKVKTVFKNEKRIKLQIWDTAGQERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWSTQIKTYSWDNAQVILVGNKCDMEDERVISTERGQHLGEQLGFEFFETSAKDNINVKQTFERLVDIICDKMSESLETDPAITAAKQNTRLKETPPPPQPNCAC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB3C, member RAS oncogene family
别名
计算分子量 26 kDa
观测分子量 28 kDa
GenBank蛋白编号BC013033
基因名称 RAB3C
Gene ID (NCBI) 115827
RRIDAB_2177217
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96E17
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Rab proteins are small molecular weight GTPases that control vesicular traffic in eukaryotic cells. A subset of Rab proteins, the Rab3 proteins (Rab3A, Rab3B, Rab3C, and Rab3D) are thought to play an important role in regulated exocytosis of vesicles. Although Rab3 proteins are highly homologous, displaying 77-85% amino acid identity with greatest variance evident within the N- and C-terminal regions, their subcellular targets and functional roles remain somewhat distinct between them. RT-PCR analysis indicated that human Rab3C was expressed in the human brain, placenta, and lung.

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