RAB3C Polyclonal antibody

RAB3C Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 10788-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA and More (1)

RAB3C, Ras related protein Rab 3C

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, mouse lung tissue, rat brain tissues
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10788-1-AP targets RAB3C in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IF, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 RAB3C fusion protein Ag1241 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB3C, member RAS oncogene family
别名 RAB3C, Ras related protein Rab 3C
计算分子量 26 kDa
观测分子量 28 kDa
GenBank蛋白编号BC013033
基因名称 RAB3C
Gene ID (NCBI) 115827
RRIDAB_2177217
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96E17
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab proteins are small molecular weight GTPases that control vesicular traffic in eukaryotic cells. A subset of Rab proteins, the Rab3 proteins (Rab3A, Rab3B, Rab3C, and Rab3D) are thought to play an important role in regulated exocytosis of vesicles. Although Rab3 proteins are highly homologous, displaying 77-85% amino acid identity with greatest variance evident within the N- and C-terminal regions, their subcellular targets and functional roles remain somewhat distinct between them. RT-PCR analysis indicated that human Rab3C was expressed in the human brain, placenta, and lung.

实验方案

Product Specific Protocols
WB protocol for RAB3C antibody 10788-1-APDownload protocol
IHC protocol for RAB3C antibody 10788-1-APDownload protocol
IP protocol for RAB3C antibody 10788-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Proc Natl Acad Sci U S A

The GTPase Rab3b/3c-positive recycling vesicles are involved in cross-presentation in dendritic cells.

Authors - Zou Liyun L
mouseIF

PLoS Genet

Cell fate specification in the lingual epithelium is controlled by antagonistic activities of Sonic hedgehog and retinoic acid.

Authors - Maha El Shahawy
humanWB

Front Endocrinol (Lausanne)

Clinical identification of expressed proteins in adrenal medullary hyperplasia detected with hypertension

Authors - He Ma
humanIHC

Signal Transduct Target Ther

Integrative analysis of DNA methylation and gene expression identified cervical cancer-specific diagnostic biomarkers.

Authors - Wanxue Xu
humanIHC

Signal Transduct Target Ther

Integrative analysis of DNA methylation and gene expression identified cervical cancer-specific diagnostic biomarkers

Authors - Wanxue Xu
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