验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human brain tissue, mouse lung tissue, rat brain tissues |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10788-1-AP targets RAB3C in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | RAB3C fusion protein Ag1241 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB3C, member RAS oncogene family |
| 别名 | RAB3C, Ras related protein Rab 3C |
| 计算分子量 | 26 kDa |
| 观测分子量 | 28 kDa |
| GenBank蛋白编号 | BC013033 |
| 基因名称 | RAB3C |
| Gene ID (NCBI) | 115827 |
| RRID | AB_2177217 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96E17 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Rab proteins are small molecular weight GTPases that control vesicular traffic in eukaryotic cells. A subset of Rab proteins, the Rab3 proteins (Rab3A, Rab3B, Rab3C, and Rab3D) are thought to play an important role in regulated exocytosis of vesicles. Although Rab3 proteins are highly homologous, displaying 77-85% amino acid identity with greatest variance evident within the N- and C-terminal regions, their subcellular targets and functional roles remain somewhat distinct between them. RT-PCR analysis indicated that human Rab3C was expressed in the human brain, placenta, and lung.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB3C antibody 10788-1-AP | Download protocol |
| IHC protocol for RAB3C antibody 10788-1-AP | Download protocol |
| IP protocol for RAB3C antibody 10788-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A The GTPase Rab3b/3c-positive recycling vesicles are involved in cross-presentation in dendritic cells. | ||
PLoS Genet Cell fate specification in the lingual epithelium is controlled by antagonistic activities of Sonic hedgehog and retinoic acid. | ||
Front Endocrinol (Lausanne) Clinical identification of expressed proteins in adrenal medullary hyperplasia detected with hypertension | ||
Signal Transduct Target Ther Integrative analysis of DNA methylation and gene expression identified cervical cancer-specific diagnostic biomarkers. | ||
Signal Transduct Target Ther Integrative analysis of DNA methylation and gene expression identified cervical cancer-specific diagnostic biomarkers |
