CoraLite® Plus 488-conjugated RAB3A Monoclonal antibody
RAB3A Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse, rat, pig, rabbit, chicken
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1B12E9
验证数据展示
经过测试的应用
| Positive FC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 68052-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68052 targets RAB3A in FC (Intra) applications and shows reactivity with Human, mouse, rat, pig, rabbit, chicken samples.
| Tested Applications | FC (Intra) |
| Tested Reactivity | Human, mouse, rat, pig, rabbit, chicken |
| Immunogen | RAB3A fusion protein Ag8148 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | RAB3A, member RAS oncogene family |
| Synonyms | RAB3A, Ras related protein Rab 3A |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC011782 |
| Gene Symbol | RAB3A |
| Gene ID (NCBI) | 5864 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P20336 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Rab3A is a small G-protein of the Rab family, that is implicated in vesicle fusion and regulated secretion, particularly in neurotransmitter release. Rab3 subfamily small G proteins (Rab3A, Rab3B, Rab3C, and Rab3D) control the regulated exocytosis in neuronal/secretory cells. Rab3A is the most abundant Rab GTPase in brain, where it is associated with synaptic vesicles. Rab3a is believed to modulate secretion efficiency by stimulating vesicle recruitment to sites of exocytosis and/ or by recruiting regulatory molecules to the docking/ fusion machinery.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 RAB3A antibody CL488-68052 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
