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验证数据展示

  • WB analysis using 15485-1-AP

    WB analysis using 15485-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15485-1-AP (RAB31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 15485-1-AP

    WB analysis of HeLa using 15485-1-AP

    WB result of RAB31 antibody (15485-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB31 transfected HeLa cells.

  • IHC staining of human liver cancer using 15485-1-AP

    IHC staining of human liver cancer using 15485-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15485-1-AP (RAB31 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB31 antibody included
  • IHC staining of human liver cancer using 15485-1-AP

    IHC staining of human liver cancer using 15485-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15485-1-AP (RAB31 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB31 antibody included
  • IHC staining of human placenta using 15485-1-AP

    IHC staining of human placenta using 15485-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15485-1-AP (RAB31 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB31 antibody included
  • IP experiment of K-562 using 15485-1-AP

    IP experiment of K-562 using 15485-1-AP

    IP result of anti-RAB31 (IP:15485-1-AP, 4ug; Detection:15485-1-AP 1:1000) with K-562 cells lysate 1640 ug.

  • Featured Product
  • KD/KO验证

RAB31 Polyclonal antibody

RAB31 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 15485-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA and More (1)

别名

RAB22B, Ras related protein Rab 31, Ras-related protein Rab-22B, Ras-related protein Rab-31

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, human placenta tissue, HepG2 cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman liver cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RAB31 Ready-To-Use IHC Kit. RAB31 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15485-1-AP targets RAB31 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag7833

Product name: Recombinant human RAB31 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-195 aa of BC001148

Sequence: MMAIRELKVCLLGDTGVGKSSIVCRFVQDHFDHNISPTIGASFMTKTVPCGNELHKFLIWDTAGQERFHSLAPMYYRGSAAAVIVYDITKQDSFYTLKKWVKELKEHGPENIVMAIAGNKCDLSDIREVPLKDAKEYAESIGAIVVETSAKNAINIEELFQGISRQIPPLDPHENGNNGTIKVEKPTMQASRRCC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB31, member RAS oncogene family
别名 RAB22B, Ras related protein Rab 31, Ras-related protein Rab-22B, Ras-related protein Rab-31
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC001148
基因名称 RAB31
Gene ID (NCBI) 11031
RRIDAB_2176911
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13636
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB31, also named as RAB22b, belongs to small GTP-binding proteins of the RAB family. Rab31 localizes in the trans-Golgi network (TGN) and endosomes. It is required for transport of mannose 6-phosphate receptors (MPRs) from the trans-Golgi network (TGN) to endosomes.

实验方案

Product Specific Protocols
WB protocol for RAB31 antibody 15485-1-APDownload protocol
IHC protocol for RAB31 antibody 15485-1-APDownload protocol
IP protocol for RAB31 antibody 15485-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Transl Med

From Bowen disease to cutaneous squamous cell carcinoma: eight markers were verified from transcriptomic and proteomic analyses

Authors - Tang Biao
View Article
humanWB,IF

J Extracell Biol

RAB31 drives extracellular vesicle fusion and cancer-associated fibroblast formation leading to oxaliplatin resistance in colorectal cancer

Authors - Yu-Chan Chang
View Article