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验证数据展示

  • IF Staining of HeLa using CL594-66058

    IF Staining of HeLa using CL594-66058

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-66058 (RAB27A antibody) at dilution of 1:100.

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  • KD/KO验证

CoraLite®594-conjugated RAB27A Monoclonal antibody

RAB27A Monoclonal Antibody for IF/ICC
Cat No. CL594-66058

产品说明书

CloneNo. 1C4B8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC

别名

GS2, GTP binding protein Ram, HsT18676, Rab 27, RAB27, RAB27A, RAM, Ras related protein Rab 27A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAB27A Ready-To-Use IHC Kit. RAB27A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66058-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66058 targets RAB27A in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 RAB27A fusion protein Ag12382 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 RAB27A, member RAS oncogene family
别名 GS2, GTP binding protein Ram, HsT18676, Rab 27, RAB27, RAB27A, RAM, Ras related protein Rab 27A
计算分子量 221 aa, 25 kDa
观测分子量 27 kDa
GenBank蛋白编号BC107680
基因名称 RAB27A
Gene ID (NCBI) 5873
RRIDAB_2883496
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP51159
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab27a is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27a is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27a is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.

实验方案

Product Specific Protocols
IF protocol for CL594 RAB27A antibody CL594-66058Download protocol
Standard Protocols
Click here to view our Standard Protocols