验证数据展示
经过测试的应用
| Positive WB detected in | mouse pancreas tissue |
| Positive IP detected in | mouse pancreas tissue |
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells, BxPC-3 cells |
| Positive FC (Intra) detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14284-1-AP targets RAB26 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag5581 Product name: Recombinant human RAB26 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-256 aa of BC066913 Sequence: MSRKKTPKSKGASTPAASTLPTANGARPARSGTALSGPDAPPNGPLQPGRPSLGGGVDFYDVAFKVMLVGDSGVGKTCLLVRFKDGAFLAGTFISTVGIDFRNKVLDVDGVKVKLQMWDTAGQERFRSVTHAYYRDAHALLLLYDVTNKASFDNIQAWLTEIHEYAQHDVALMLLGNKVDSAHERVVKREDGEKLAKEYGLPFMETSAKTGLNVDLAFTAIAKELKRRSMKAPSEPRFRLHDYVKREGRGASCCRP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB26, member RAS oncogene family |
| 别名 | Ras related protein Rab 26, Ras-related protein Rab-26 |
| 计算分子量 | 28 kDa |
| 观测分子量 | 27-30 kDa |
| GenBank蛋白编号 | BC066913 |
| 基因名称 | RAB26 |
| Gene ID (NCBI) | 25837 |
| RRID | AB_2176709 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9ULW5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for RAB26 antibody 14284-1-AP | Download protocol |
| IF protocol for RAB26 antibody 14284-1-AP | Download protocol |
| IHC protocol for RAB26 antibody 14284-1-AP | Download protocol |
| IP protocol for RAB26 antibody 14284-1-AP | Download protocol |
| WB protocol for RAB26 antibody 14284-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Genes Dis RAB26 promotes prostate cancer progression via the MAPK/ERK-TWIST1 signaling axis | ||
Cell Death Dis SNRPB promotes the tumorigenic potential of NSCLC in part by regulating RAB26.
| ||
Cell Death Dis Rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated Src. | ||
Sci Total Environ Manganese disrupts the maturation and degradation of axonal autophagosome leading to hippocampal synaptic toxicity in mice via the activation of LRRK2 on phosphorylation of Rab10 | ||
Free Radic Biol Med ROS induced the Rab26 promoter hypermethylation to promote cigarette smoking-induced airway epithelial inflammation of COPD through activation of MAPK signaling
| ||
