验证数据展示
经过测试的应用
Positive WB detected in | mouse pancreas tissue |
Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells, BxPC-3 cells |
Positive FC (Intra) detected in | MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14284-1-AP targets RAB26 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse |
免疫原 | RAB26 fusion protein Ag5581 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | RAB26, member RAS oncogene family |
别名 | Ras-related protein Rab-26, Ras related protein Rab 26 |
计算分子量 | 28 kDa |
观测分子量 | 27-30 kDa |
GenBank蛋白编号 | BC066913 |
基因名称 | RAB26 |
Gene ID (NCBI) | 25837 |
RRID | AB_2176709 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9ULW5 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
---|---|
WB protocol for RAB26 antibody 14284-1-AP | Download protocol |
IHC protocol for RAB26 antibody 14284-1-AP | Download protocol |
IF protocol for RAB26 antibody 14284-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Total Environ Manganese-induced α-synuclein overexpression promotes the accumulation of dysfunctional synaptic vesicles and hippocampal synaptotoxicity by suppressing Rab26-dependent autophagy in presynaptic neurons | ||
Cell Death Dis SNRPB promotes the tumorigenic potential of NSCLC in part by regulating RAB26.
| ||
Cell Death Dis Rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated Src. | ||
J Immunol Res Novel Circulating Tumour Cell-Related Risk Model Indicates Prognosis and Immune Infiltration in Lung Adenocarcinoma.
| ||
Free Radic Biol Med ROS induced the Rab26 promoter hypermethylation to promote cigarette smoking-induced airway epithelial inflammation of COPD through activation of MAPK signaling
|