验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, Jurkat cells, BxPC-3 cells, A375 cells |
| Positive IP detected in | COLO 320 cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12125-1-AP targets RAB22A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, canine, hamster |
| 免疫原 | RAB22A fusion protein Ag2769 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB22A, member RAS oncogene family |
| 别名 | Rab 22, RAB22, RAB22A, Ras related protein Rab 22A |
| 计算分子量 | 194 aa, 22 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC015710 |
| 基因名称 | RAB22A |
| Gene ID (NCBI) | 57403 |
| RRID | AB_2173765 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UL26 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAB22A is a small GTPase that is expressed ubiquitously in mammalian tissues. It localizes in the MHCI-containing recycling endosomal tubules and functions in endocytic pathway. RAB22A may regulate the dynamic interactions of endosomal compartments and it may be involved in the communication between the biosynthetic and early endocytic pathways. It shares 81% identity with RAB31.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB22A antibody 12125-1-AP | Download protocol |
| IHC protocol for RAB22A antibody 12125-1-AP | Download protocol |
| IP protocol for RAB22A antibody 12125-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Clin Cancer Res The Immune-microenvironment Confers Chemoresistance of Colorectal Cancer through Macrophage-derived IL-6. | ||
EMBO Rep Rab22A recruits BLOC-1 and BLOC-2 to promote the biogenesis of recycling endosomes.
| ||
J Cell Sci A specific subset of RabGTPases controls cell surface exposure of MT1-MMP, extracellular matrix degradation and three-dimensional invasion of macrophages. | ||
Traffic Rab GTPases regulating phagosome maturation are differentially recruited to mycobacterial phagosomes. | ||
Mol Biol Cell Rabex-5 is a Rab22 effector and mediates a Rab22-Rab5 signaling cascade in endocytosis.
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