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验证数据展示

  • WB analysis using 11718-1-AP

    WB analysis using 11718-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11718-1-AP (RAB13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 11718-1-AP

    WB analysis of HEK-293 using 11718-1-AP

    WB result of RAB13 antibody (11718-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB13 transfected HEK-293 cells.

  • FC experiment of K-562 using 11718-1-AP

    FC experiment of K-562 using 11718-1-AP

    1x10^6 K-562 cells were intracellularly stained with 0.4 ug RAB13 Polyclonal antibody (11718-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IHC staining of mouse testis using 11718-1-AP

    IHC staining of mouse testis using 11718-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11718-1-AP (RAB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAB13 antibody included
  • IF Staining of HeLa using 11718-1-AP

    IF Staining of HeLa using 11718-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB13 antibody (11718-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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RAB13 Polyclonal antibody

RAB13 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11718-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

GIG4, Cell growth inhibiting gene 4 protein, Cell growth-inhibiting gene 4 protein, Ras related protein Rab 13, Ras-related protein Rab-13

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, mouse colon tissue, mouse lung tissue, rat lung tissue, rat colon tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy RAB13 Ready-To-Use IHC Kit. RAB13 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11718-1-AP targets RAB13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2292

Product name: Recombinant human RAB13 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-203 aa of BC000799

Sequence: MAKAYDHLFKLLLIGDSGVGKTCLIIRFAEDNFNNTYISTIGIDFKIRTVDIEGKKIKLQVWDTAGQERFKTITTAYYRGAMGIILVYDITDEKSFENIQNWMKSIKENASAGVERLLLGNKCDMEAKRKVQKEQADKLAREHGIRFFETSAKSSMNVDEAFSSLARDILLKSGGRRSGNGNKPPSTDLKTCDKKNTNKCSLG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAB13, member RAS oncogene family
别名 GIG4, Cell growth inhibiting gene 4 protein, Cell growth-inhibiting gene 4 protein, Ras related protein Rab 13, Ras-related protein Rab-13
计算分子量 203 aa, 23 kDa
观测分子量 23 kDa
GenBank蛋白编号BC000799
基因名称 RAB13
Gene ID (NCBI) 5872
RRIDAB_3669132
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51153
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB13 (Ras-related protein Rab-13) is also named as GIG4. Rab13 is a member of the Rab GTPase family. RAB13 is highly expressed in malignant cells. Rab13 was highly expressed in BCSC of triple-negative breast cancer (TNBC) (PMID: 35395074). RAB13 is involved in a number of processes in LIHC, including MTORC1 signaling, MYC targets v1, G2M checkpoint, MITOTIC spindle, DNA repair, P53 pathway, glycolysis, PI3K-AKT-MTOR signaling, etc (PMID: 37803063). RAB13 enhances its circulation to the plasma membrane to promote breast cancer invasion. By influencing the tumor microenvironment, RAB13 maintains breast cancer stem cells (PMID: 37803063).

实验方案

Product Specific Protocols
IF protocol for RAB13 antibody 11718-1-APDownload protocol
IHC protocol for RAB13 antibody 11718-1-APDownload protocol
WB protocol for RAB13 antibody 11718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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