验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-18136 targets RAB11FIP2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag12736 Product name: Recombinant human RAB11FIP2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-373 aa of BC075073 Sequence: MMLSEQAQKWFPTHVQVTVLQAKDLKPKGKSGTNDTYTIIQLGKEKYSTSVAEKTLEPVWKEEASFELPGLLIQGSPEKYILFLIVMHRSLVGLDKFLGQVAINLNDIFEDKQRRKTEWFRLESKQGKRIKNRGEIKVNIQFMRNNMTASMFDLSMKDKTRSPFAKLKDKMKGRKNDGTFSDTSSAIIPSTHMPDANSEFSSGEIQMKSKPKKPFLLGPQRLSSAHSMSDLSGSHMSSEKLKAGTIGQTHLLGHQLDSFGTVPESGSLKSPHRRTLSFDTSKMNQPDSIVDEGELCFGRQNDPFTNVTASLPQKFATLPRKKNPFEESSETWDSSMNLFSKPIEIRKENKREKREKVSLFERVTGKKDSRRSD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB11 family interacting protein 2 (class I) |
| 别名 | NRip11, Rab11 family-interacting protein 2, Rab11 FIP2, Rab11-FIP2 |
| 计算分子量 | 512 aa, 58 kDa |
| 观测分子量 | 58 kDa |
| GenBank蛋白编号 | BC075073 |
| 基因名称 | RAB11FIP2 |
| Gene ID (NCBI) | 22841 |
| RRID | AB_3672688 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 激发激光 | Blue laser (488 nm) |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7L804 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAB11FIP2, or RAB11 family interacting protein 2, is a protein that plays a crucial role in intracellular trafficking, particularly in the recycling of endocytic vesicles. It is involved in several cellular processes, including TRAM-dependent toll-like receptor 4 signaling pathway, phagocytosis, and positive regulation of GTPase activity. RAB11FIP2 is localized in endosomes, nucleoplasm, and phagocytic cups, and it is expressed ubiquitously in various tissues, with higher expression in endometrium and brain. RAB11FIP2 interacts with the actin-based motor myo5b and the small GTPase Rab11, which are key components in the regulation of vesicle tethering and transport along actin filaments. This interaction is particularly important for processes such as cell polarization, motility, and lumen formation in multicellular structures. .
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 RAB11FIP2 antibody CL488-18136 | Download protocol |
| IF protocol for CL Plus 488 RAB11FIP2 antibody CL488-18136 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
