验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, NIH/3T3 cells, rat brain tissue, C6 cells, HeLa cells, MCF-7 cells, MDCK cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Planning an IHC experiment? We recommend our IHCeasy RAB10 Ready-To-Use IHC Kit. RAB10 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11808-1-AP targets RAB10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat, canine |
| 文献引用反应性 | human, mouse, rat, zebrafish |
| 免疫原 | RAB10 fusion protein Ag2473 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAB10, member RAS oncogene family |
| 别名 | Ras-related protein Rab-10, Ras related protein Rab 10, EC:3.6.5.2 |
| 计算分子量 | 200 aa, 23 kDa |
| 观测分子量 | 23 kDa |
| GenBank蛋白编号 | BC000896 |
| 基因名称 | RAB10 |
| Gene ID (NCBI) | 10890 |
| RRID | AB_2173442 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P61026 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAB10 belongs to the RAS superfamily of small GTPases. RAB10 localizes to a system of tubules and vesicles, and it may be involved in vesicular trafficking and neurotransmitter release. It is also associated with Glut4 translocation in adipocytes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB10 antibody 11808-1-AP | Download protocol |
| IHC protocol for RAB10 antibody 11808-1-AP | Download protocol |
| IF protocol for RAB10 antibody 11808-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development. | ||
Nat Commun Myosin Vb controls biogenesis of post-Golgi Rab10 carriers during axon development. | ||
Proc Natl Acad Sci U S A Ras-related protein Rab10 facilitates TLR4 signaling by promoting replenishment of TLR4 onto the plasma membrane. | ||
Curr Biol Stretch triggers microtubule stabilization and MARCKS-dependent membrane incorporation in the shaft of embryonic axons | ||
Sci Signal Semaphorin 3A activates the guanosine triphosphatase Rab5 to promote growth cone collapse and organize callosal axon projections. | ||
J Neurosci Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis. |
