Prolactin Recombinant antibody

Prolactin Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 85765-3-RR

产品说明书

CloneNo. 242898A8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

PRL

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inmouse pituitary gland tissue, NIH/3T3 cells, mouse uterus tissue
Positive IHC detected inmouse pituitary gland tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85765-3-RR targets Prolactin in applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Prolactin fusion protein Ag9764 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 prolactin
别名 PRL
计算分子量 227 aa, 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC015850
基因名称 Prolactin/PRL
Gene ID (NCBI) 5617
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01236
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Prolactin is also named as PRL and belongs to the somatotropin/prolactin family. The proteins encoded by PRL are secreted into the cell surroundings. And they are abundantly expressed in pituitary gland, adenohypophysis, decidua and testis. Indeed, chemically, prolactin appears in a multiplicity of posttranslational forms ranging from size variants to chemical modifications such as phosphorylation or glycosylation. It is not only synthesized in the pituitary gland, as originally described, but also within the central nervous system, the immune system, the uterus and its associated tissues of conception, and even the mammary gland itself (PMID: 11015620). Prolactin acts primarily on the mammary gland by promoting lactation (PMID: 30546056). The major form of prolactin found in the pituitary gland is 23 kDa, variants of prolactin have been characterized in many mammals, including humans. Of the cleaved forms that have been characterized, 14 kDa, 16 kDa, and 22 kDa prolactin variants have been most widely studied (PMID: 7937959) (PMID: 8425495).

实验方案

Product Specific Protocols
WB protocol for Prolactin antibody 85765-3-RRDownload protocol
IHC protocol for Prolactin antibody 85765-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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