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验证数据展示

  • WB analysis using 85765-3-RR (same clone as 85765-3-PBS)

    WB analysis using 85765-3-RR (same clone as 85765-3-PBS)

    mouse pituitary gland tissue were subjected to SDS PAGE followed by western blot with 85765-3-RR (PRL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85765-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse pituitary gland using 85765-3-RR (same clone as 85765-3-PBS)

    IHC staining of mouse pituitary gland using 85765-3-RR (same clone as 85765-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pituitary gland tissue slide using 85765-3-RR (Prolactin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85765-3-PBS in a different storage buffer formulation.

  • IF Staining of mouse pituitary gland using 85765-3-RR (same clone as 85765-3-PBS)

    IF Staining of mouse pituitary gland using 85765-3-RR (same clone as 85765-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pituitary gland tissue using Prolactin antibody (85765-3-RR, Clone: 242898A8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85765-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85765-3-RR

    Affinity and Kinetic Characterization of 85765-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85765-3-RR against Human Prolactin were performed. The affinity constant is 0.122 nM.

Prolactin Recombinant monoclonal antibody, PBS Only

Prolactin Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 85765-3-PBS

产品说明书

CloneNo. 242898A8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

PRL

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85765-3-PBS targets Prolactin in WB, IHC, IF-P, Cytometric bead array, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag9764

Product name: Recombinant human PRL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-227 aa of BC015850

Sequence: MNIKGSPWKGSLLLLLVSNLLLCQSVAPLPICPGGAARCQVTLRDLFDRAVVLSHYIHNLSSEMFSEFDKRYTHGRGFITKAINSCHTSSLATPEDKEQAQQMNQKDFLSLIVSILRSWNEPLYHLVTEVRGMQEAPEAILSKAVEIEEQTKRLLEGMELIVSQVHPETKENEIYPVWSGLPSLQMADEESRLSAYYNLLHCLRRDSHKIDNYLKLLKCRIIHNNNC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 prolactin
别名 PRL
计算分子量 227 aa, 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC015850
基因名称 Prolactin/PRL
Gene ID (NCBI) 5617
RRIDAB_3744358
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP01236
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Prolactin is also named as PRL and belongs to the somatotropin/prolactin family. The proteins encoded by PRL are secreted into the cell surroundings. And they are abundantly expressed in pituitary gland, adenohypophysis, decidua and testis. Indeed, chemically, prolactin appears in a multiplicity of posttranslational forms ranging from size variants to chemical modifications such as phosphorylation or glycosylation. It is not only synthesized in the pituitary gland, as originally described, but also within the central nervous system, the immune system, the uterus and its associated tissues of conception, and even the mammary gland itself (PMID: 11015620). Prolactin acts primarily on the mammary gland by promoting lactation (PMID: 30546056). The major form of prolactin found in the pituitary gland is 23 kDa, variants of prolactin have been characterized in many mammals, including humans. Of the cleaved forms that have been characterized, 14 kDa, 16 kDa, and 22 kDa prolactin variants have been most widely studied (PMID: 7937959) (PMID: 8425495).

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