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验证数据展示

  • WB analysis of MCF-7 using 85112-4-RR

    WB analysis of MCF-7 using 85112-4-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 85112-4-RR (PR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85112-4-RR

    WB analysis using 85112-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85112-4-RR (Progesterone Receptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 85112-4-RR

    IHC staining of human cervical cancer using 85112-4-RR

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85112-4-RR (PR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PR antibody included
  • IHC staining of human cervical cancer using 85112-4-RR

    IHC staining of human cervical cancer using 85112-4-RR

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85112-4-RR (PR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PR antibody included
  • IF Staining of T-47D using 85112-4-RR

    IF Staining of T-47D using 85112-4-RR

    Immunofluorescent analysis of (4% PFA) fixed T-47D cells, MCF-7 cells, MDA-MB-231 cells using PR antibody (85112-4-RR, Clone: 242686G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85112-4-RR

    Affinity and Kinetic Characterization of 85112-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85112-4-RR against Human Progesterone Receptor were performed. The affinity constant is 0.587 nM.

Progesterone Receptor Recombinant antibody

Progesterone Receptor Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85112-4-RR

产品说明书

CloneNo. 242686G1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

PGR, NR3C3, PR, PRGR

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, T-47D cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inT-47D cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy PR Ready-To-Use IHC Kit. PR primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85112-4-RR targets Progesterone Receptor in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Progesterone Receptor fusion protein Ag22986 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 progesterone receptor
别名 PGR, NR3C3, PR, PRGR
计算分子量 933 aa, 99 kDa
观测分子量 118 kDa
GenBank蛋白编号NM_000926
基因名称 Progesterone Receptor
Gene ID (NCBI) 5241
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP06401
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PGR (Progesterone Receptor), also named as NR3C3 and PR, belongs to the nuclear hormone receptor family and NR3 subfamily. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. The ER and PR status has been used as a predictor of breast carcinoma responsiveness to endocrine therapy and as a prognostic indicator for early recurrence.

实验方案

Product Specific Protocols
WB protocol for Progesterone Receptor antibody 85112-4-RRDownload protocol
IHC protocol for Progesterone Receptor antibody 85112-4-RRDownload protocol
IF protocol for Progesterone Receptor antibody 85112-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols