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验证数据展示

  • WB analysis of MCF-7 using 85112-4-RR (same clone as 85112-4-PBS)

    WB analysis of MCF-7 using 85112-4-RR (same clone as 85112-4-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 85112-4-RR (PR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85112-4-PBS in a different storage buffer formulation.

  • WB analysis using 85112-4-RR (same clone as 85112-4-PBS)

    WB analysis using 85112-4-RR (same clone as 85112-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85112-4-RR (Progesterone Receptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85112-4-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 85112-4-RR (same clone as 85112-4-PBS)

    IHC staining of human cervical cancer using 85112-4-RR (same clone as 85112-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85112-4-RR (PR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85112-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PR antibody included
  • IHC staining of human cervical cancer using 85112-4-RR (same clone as 85112-4-PBS)

    IHC staining of human cervical cancer using 85112-4-RR (same clone as 85112-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85112-4-RR (PR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85112-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PR antibody included
  • IF Staining of T-47D using 85112-4-RR (same clone as 85112-4-PBS)

    IF Staining of T-47D using 85112-4-RR (same clone as 85112-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed T-47D cells, MCF-7 cells, MDA-MB-231 cells using PR antibody (85112-4-RR, Clone: 242686G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85112-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85112-4-RR

    Affinity and Kinetic Characterization of 85112-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85112-4-RR against Human Progesterone Receptor were performed. The affinity constant is 0.587 nM.

Progesterone Receptor Recombinant antibody, PBS Only

Progesterone Receptor Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85112-4-PBS

产品说明书

CloneNo. 242686G1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

PGR, NR3C3, PR, PRGR

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PR Ready-To-Use IHC Kit. PR primary antibody included.

产品信息

85112-4-PBS targets Progesterone Receptor in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Progesterone Receptor fusion protein Ag22986 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 progesterone receptor
别名 PGR, NR3C3, PR, PRGR
计算分子量 933 aa, 99 kDa
观测分子量 118 kDa
GenBank蛋白编号NM_000926
基因名称 Progesterone Receptor
Gene ID (NCBI) 5241
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP06401
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PGR (Progesterone Receptor), also named as NR3C3 and PR, belongs to the nuclear hormone receptor family and NR3 subfamily. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. The ER and PR status has been used as a predictor of breast carcinoma responsiveness to endocrine therapy and as a prognostic indicator for early recurrence.