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验证数据展示

  • WB analysis using 86577-1-RR (same clone as 86577-1-PBS)

    WB analysis using 86577-1-RR (same clone as 86577-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86577-1-RR (Pon3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86577-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02543-1

    Sandwich ELISA sample test of MP02543-1

    The mean PON3 concentration was determined to be 41.6 ng/mL in RAW 264.7 cell extract based on a 1.3 mg/mL extract load and 1,033.8 ng/mL in mouse liver tissue extract based on a 8.4 mg/mL extract load.

  • Sandwich ELISA sample test of MP02543-1

    Sandwich ELISA sample test of MP02543-1

    Serum of six mice was measured. The PON3 concentration of detected samples was determined to be 19.6 ng/mL with a range of 16.7-21.2 ng/mL.

  • Sandwich ELISA standard curve of MP02543-1

    Sandwich ELISA standard curve of MP02543-1

    Sandwich ELISA standard curve of MP02543-1, Mouse PON3 Recombinant Matched Antibody Pair - PBS only. 86577-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2743. 86577-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Cytometric bead array sample test of MP02543-1

    Cytometric bead array sample test of MP02543-1

    The mean PON3 concentration was determined to be 986.3 ng/mL in Mouse liver tissue extract based on a 8.4 mg/mL extract load.

  • Cytometric bead array standard curve of MP02543-1

    Cytometric bead array standard curve of MP02543-1

    Cytometric bead array standard curve of MP02543-1, MOUSE PON3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86577-2-PBS. Detection antibody: 86577-1-PBS. Standard: Eg2743. Range: 0.469-60 ng/mL.

Mouse Pon3 Recombinant monoclonal antibody, PBS Only (Detector)

Pon3 Uni-rAb® Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 86577-1-PBS

产品说明书

CloneNo. 251535H2

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

别名

EC:3.1.8.1, Paraoxanase-3, Serum paraoxonase/lactonase 3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86577-1-PBS targets Pon3 as part of a matched antibody pair:

MP02543-1: 86577-2-PBS capture and 86577-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg2743

Product name: Recombinant Mouse Pon3 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 20-354 aa of NM_173006.1

Sequence: RLLNFRERVSTTREIKATEPQNCHLIEGLENGSEDIDILPSGLAFISTGLKYPGMPAFAPDKPGRIFLMDLNEQNPEAQALEISGGLDQESLNPHGISTFIDKDNTAYLYVVNHPNMDSTVEIFKFEEQQRSLIHLKTLKHELLKSVNDIVVLGPEQFYATRDHYFTSYFLVLLEMILDPHWTSVVFYSPKEVKVVAQGFSSANGITVSLDQKFVYVADVTAKNIHIMKKHDNWDLTPVKVIQLGTLVDNLTVDPATGDILAGCHPNPMKLLIYNPEDPPGSEVLRIQDSLSDKPRVSTLYANNGSVLQGSTVASVYHKRMLIGTIFHKALYCDL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 paraoxonase 3
别名 EC:3.1.8.1, Paraoxanase-3, Serum paraoxonase/lactonase 3
计算分子量39 kDa
观测分子量40 kDa
GenBank蛋白编号NM_173006.1
基因名称 Pon3
Gene ID (NCBI) 269823
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ62087
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PON3 (paraoxonase 3) is a member of the paraoxonase gene family and shares high homology with PON1 and PON2. The PON3 protein is primarily synthesized by the liver and can bind to high-density lipoprotein (HDL), where it exerts antioxidant functions. PON3 can prevent the oxidation of low-density lipoprotein (LDL) and disrupt bacterial quorum-sensing molecules. Additionally, PON3 is expressed in mouse models of Alzheimer's disease (AD), with positive staining for PON3 detected in astrocytes surrounding Aβ plaques. These findings suggest that PON3 plays an important role in antioxidant stress and lipid peroxidation and may have potential protective effects on pathological processes such as neurodegenerative diseases and atherosclerosis.

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