验证数据展示
经过测试的应用
| Positive WB detected in | IGF-1 treated MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 1 publications below |
产品信息
82373-1-RR targets Phospho-p70(S6K) (Thr389)/p85(S6K) (Thr412) in WB, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ribosomal protein S6 kinase, 70kDa, polypeptide 1 |
| 别名 | p p70(S6K), p p70(S6K) (Thr389), p p70(S6K) Thr389, P p85(S6K) (Thr412), p70(S6K) (Thr389) |
| 观测分子量 | 65-85 kDa |
| GenBank蛋白编号 | NM_003161 |
| 基因名称 | p70 S6K |
| Gene ID (NCBI) | 6198 |
| RRID | AB_3086477 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P23443 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Rps6kb1 gene encodes the 70 kDa ribosomal protein S6 kinase (p70S6K), which is a serine/threonine kinase regulated by phosphoinositide 3-kinase (PI3K)/mammalian target of rapamycin (mTOR) pathway. P70(S6K) plays a crucial role in controlling cell cycle, growth and survival. The PI3K/mTOR signalling pathway is one of the major mechanisms for controlling cell survival, proliferation and metabolism and is the central regulator of translation of some components of protein synthesis system. Due to alternative translation two isoform S6K1 proteins are known to exist in mammalian cells: p85(S6K) and p70(S6K), which is identical to p85(S6K) but lacks its first 23 amino acids. In addition, mammalian cells express a second S6K1 isoform spanning 316 amino acids (p31 S6K1). mTOR is know to phosphorylate and thereby activate p70(S6K) at Thr389 and p85(S6K) at Thr412. (PMID: 25100792, PMID: 24970012, PMID: 21602892)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-p70(S6K) (Thr389)/p85(S6K) (Thr412) antibody 82373-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci SCAMP3-Driven Regulation of ERK1/2 and Autophagy Phosphoproteomics Signatures in Triple-Negative Breast Cancer. | ||
Elife Mycobacterial metallophosphatase MmpE acts as a nucleomodulin to regulate host gene expression and promote intracellular survival. | ||
Free Radic Biol Med Bufotenidine attenuates astrocyte ferroptosis in depressive-like behaviors through targeting GluA1 to reduce neuronal lipid synthesis. | ||
Cell Death Dis LINC00622 transcriptionally promotes RRAGD to repress mTORC1-modulated autophagic cell death by associating with BTF3 in cutaneous melanoma | ||
Int J Biol Macromol Autophagic damage in senescent bone marrow mesenchymal stromal cells: Impact on Piezo1 expression during osteoporosis progression | ||
Cell Biochem Biophys Grape Seed Proanthocyanins Extract Ameliorates the Neurotoxicity Induced by Fluoride Through mTOR/p70s6k Signaling Pathway in Vivo and in Vitro |
