验证数据展示
经过测试的应用
| Positive WB detected in | IGF-1 treated MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28735-1-AP targets Phospho-p70(S6K) (Thr389)/p85(S6K) (Thr412) in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, mouse, rat, pig, bovine |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein S6 kinase, 70kDa, polypeptide 1 |
| 别名 | p p70(S6K), p p70(S6K) (Thr389), p p70(S6K) Thr389, p70 S6 Kinase (Thr389), p70(S6K) (Thr389) |
| 观测分子量 | 65-85 kDa |
| GenBank蛋白编号 | NM_003161 |
| 基因名称 | p70 S6K |
| Gene ID (NCBI) | 6198 |
| RRID | AB_2918197 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P23443 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Rps6kb1 gene encodes the 70 kDa ribosomal protein S6 kinase (p70S6K), which is a serine/threonine kinase regulated by phosphoinositide 3-kinase (PI3K)/mammalian target of rapamycin (mTOR) pathway. P70(S6K) plays a crucial role in controlling cell cycle, growth and survival. The PI3K/mTOR signalling pathway is one of the major mechanisms for controlling cell survival, proliferation and metabolism and is the central regulator of translation of some components of protein synthesis system. Due to alternative translation two isoform S6K1 proteins are known to exist in mammalian cells: p85(S6K) and p70(S6K), which is identical to p85(S6K) but lacks its first 23 amino acids. In addition, mammalian cells express a second S6K1 isoform spanning 316 amino acids (p31 S6K1). mTOR is know to phosphorylate and thereby activate p70(S6K) at Thr389 and p85(S6K) at Thr412. (PMID: 25100792, PMID: 24970012, PMID: 21602892)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-p70(S6K) (Thr389)/p85(S6K) (Thr412) antibody 28735-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Psychological stress drives aging-like hematopoietic stem cell dysfunction through a brain-gut-bone marrow axis. | ||
Cancer Res ASS1-mediated reductive carboxylation of cytosolic glutamine confers ferroptosis resistance in cancer cells | ||
Drug Resist Updat Methylation of GPRC5A promotes liver metastasis and docetaxel resistance through activating mTOR signaling pathway in triple negative breast cancer | ||
Drug Resist Updat Host-directed novel mechanistic insights of doxorubicin reveal its efficacy against drug-resistant HSV-1 underscoring risks with oncolytic virotherapy. | ||
Autophagy PSAT1 inhibits mTORC1 activation by preventing Rag heterodimer formation in lung adenocarcinoma | ||
Autophagy FGF21 rejuvenates aged human adipose-derived mesenchymal stem cells via enhancement of TFE3-mediated autophagy flux. |
