CoraLite® Plus 488-conjugated Phospho-mTOR (Ser2448) Recombinant antibody
Phospho-mTOR (Ser2448) Recombinant Antibody for FC (Intra), IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
Human, Rat
Applications
FC (Intra), IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3L18
验证数据展示
经过测试的应用
Positive IF detected in | PMA treated HEK-293 cells |
Positive FC detected in | Calyculin A treated HeLa cells, HeLa cells |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-80596 targets Phospho-mTOR (Ser2448) in IF, FC (Intra) applications and shows reactivity with Human, Rat samples.
Tested Applications | FC (Intra), IF/ICC |
Tested Reactivity | Human, Rat |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | FK506 binding protein 12-rapamycin associated protein 1 |
Synonyms | FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, Phospho-mTOR (Ser2448), RAFT1, Rapamycin target protein 1, RAPT1 |
Calculated Molecular Weight | 289 kDa |
Observed Molecular Weight | 250-289 kDa |
GenBank Accession Number | BC117166 |
Gene Symbol | FRAP1 |
Gene ID (NCBI) | 2475 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 Phospho-mTOR (Ser2448) antibody CL488-80596 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |