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验证数据展示

  • WB analysis using 80351-2-RR (same clone as 80351-2-PBS)

    WB analysis using 80351-2-RR (same clone as 80351-2-PBS)

    HeLa cell lysates were subjected to SDS PAGE followed by western blot with 80351-2-RR (Phospho-eEF2K (Thr57) antibody) blocked with BSA only, Phospho-eEF2K (Thr57) peptide or non-Phospho peptide at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80351-2-PBS in a different storage buffer formulation.

Phospho-eEF2K (Thr57) Recombinant antibody, PBS Only

Phospho-eEF2K (Thr57) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 80351-2-PBS

产品说明书

CloneNo. 250222A6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

EEF2K, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, EC:2.7.11.20, eEF 2 kinase, eEF 2K

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

80351-2-PBS targets Phospho-eEF2K (Thr57) in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 eukaryotic elongation factor-2 kinase
别名 EEF2K, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, EC:2.7.11.20, eEF 2 kinase, eEF 2K
计算分子量 725 aa, 100 kDa
观测分子量100 kDa
GenBank蛋白编号BC032665
基因名称 EEF2K
Gene ID (NCBI) 29904
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO00418
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Eukaryotic elongation factor‐2 kinase (eEF2K), well‐known to be a Ca2+ calmodulin (CaM)‐dependent kinase, is overexpressed in several types of malignancies, most notably Triple negative breast cancer (TNBC). Increasing evidence indicates that eEF2K may modulate the expression of some apoptotic proteins such as XIAP, c‐FLIPL, Bcl‐XL, PI3KCI and p70S6K to inhibit apoptotic process in cancer. On the other hand, it may regulate autophagy involved in AMPK, mTORC1 and ULK1, thereby promoting cancer cell survival. (PMID: 29094413)