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验证数据展示

  • WB analysis of HepG2 using 80694-2-RR

    WB analysis of HepG2 using 80694-2-RR

    Calyculin A treated and untreated HepG2 cells were subjected to SDS PAGE followed by western blot with 80694-2-RR (Phospho-YAP1 (Ser127) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 80694-2-RR

    FC experiment of HepG2 using 80694-2-RR

    1X10^6 HepG2 cells untreated (dashed lines) or treated with Calyculin A intracellularly stained with 0.13 ug Phospho-YAP1 (Ser127) Recombinant antibody (80694-2-RR, Clone:240779G4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-YAP1 (Ser127) Recombinant antibody

Phospho-YAP1 (Ser127) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 80694-2-RR

产品说明书

CloneNo. 240779G4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

Phospho YAP1, p YAP1 Ser127, p YAP1 (Ser127), p YAP1, Phospho YAP1 (Ser127)

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCalyculin A treated HepG2 cells
Positive FC (Intra) detected inCalyculin A treated HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy YAP1 Ready-To-Use IHC Kit. YAP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80694-2-RR targets Phospho-YAP1 (Ser127) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Yes-associated protein 1, 65kDa
别名 Phospho YAP1, p YAP1 Ser127, p YAP1 (Ser127), p YAP1, Phospho YAP1 (Ser127)
计算分子量 504 aa, 54 kDa
观测分子量70-78 kDa
GenBank蛋白编号BC038235
基因名称 YAP1
Gene ID (NCBI) 10413
RRIDAB_3670484
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP46937
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The transcriptional factors Yes-associated protein 1 (YAP1) and PDZ-binding motif (TAZ) act as downstream effectors of the Hippo pathway, and their subcellular location and transcriptional activities are affected by multiple post-translational modifications (PTMs). After phosphorylation, YAP1/TAZ binds to the 14-3-3 protein, which induces cytoplasmic retention of YAP1/TAZ. Moreover, phosphorylated YAP1/TAZ is likely to undergo ubiquitination degradation that is dependent on the β-transducin repeat-containing E3 ubiquitin protein ligase complex. The phosphorylation of YAP at Ser127 promotes its cytoplasmic retention, whereas phosphorylation at Ser397 induces degradation. (PMID: 29682330, PMID: 26039999)

实验方案

Product Specific Protocols
WB protocol for Phospho-YAP1 (Ser127) antibody 80694-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols