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验证数据展示

  • WB analysis using 87149-1-RR

    WB analysis using 87149-1-RR

    Non-treated HeLa cells and paclitaxel treated HeLa cells were subjected to SDS PAGE followed by western blot with 87149-1-RR (Phospho-Vimentin (Ser56) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • WB analysis using 87149-1-RR

    WB analysis using 87149-1-RR

    Non-treated Jurkat cells and paclitaxel treated Jurkat cells were subjected to SDS PAGE followed by western blot with 87149-1-RR (Phospho-Vimentin (Ser56) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • FC experiment of Jurkat using 87149-1-RR

    FC experiment of Jurkat using 87149-1-RR

    1x10^6 Jurkat cells untreated (dashed lines) or treated with paclitaxel which intracellularly stained with 0.13 ug Phospho-Vimentin (Ser56) Recombinant monoclonal antibody (87149-1-RR, Clone:252334B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Phospho-Vimentin (Ser56) Recombinant monoclonal antibody

Phospho-Vimentin (Ser56) Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 87149-1-RR

产品说明书

CloneNo. 252334B3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

Phospho Vimentin, Phospho Vimentin (Ser56), Phospho-VIM (Ser56), Phospho-Vimentin, Phospho-Vimentin(Ser56)

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inpaclitaxel treated Jurkat cells, paclitaxel treated HeLa cells
Positive FC (Intra) detected inpaclitaxel treated Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy Vimentin Ready-To-Use IHC Kit. Vimentin primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87149-1-RR targets Phospho-Vimentin (Ser56) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 vimentin
别名 Phospho Vimentin, Phospho Vimentin (Ser56), Phospho-VIM (Ser56), Phospho-Vimentin, Phospho-Vimentin(Ser56)
计算分子量 54 kDa
观测分子量57 kDa
GenBank蛋白编号NM_003380
基因名称 VIM
Gene ID (NCBI) 7431
ENSEMBL Gene IDENSG00000026025
RRIDAB_3745371
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP08670
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vimentin, also named as VIM, belongs to the intermediate filament family. Phospho-Vimentin (Ser56) is a phosphorylated form of the intermediate filament protein Vimentin that plays a significant role in regulating cellular functions such as smooth muscle contraction, cell migration, and mitosis. Phosphorylation of Vimentin at Ser-56 has been found to regulate vimentin depolymerization and the spatial reorientation of vimentin filaments, which modulates intercellular force transmission and contraction in smooth muscle. (PMID: 27457922)

实验方案

Product Specific Protocols
FC protocol for Phospho-Vimentin (Ser56) antibody 87149-1-RRDownload protocol
WB protocol for Phospho-Vimentin (Ser56) antibody 87149-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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