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验证数据展示

  • WB analysis using 80553-2-RR (same clone as 80553-2-PBS)

    WB analysis using 80553-2-RR (same clone as 80553-2-PBS)

    Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80553-2-RR (Phospho-TSC2 (Ser1387) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin antibody (66305-1-Ig) as the loading control. This data was developed using the same antibody clone with 80553-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 80553-2-RR (same clone as 80553-2-PBS)

    FC experiment of HeLa using 80553-2-RR (same clone as 80553-2-PBS)

    1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-TSC2 (Ser1387) Recombinant monoclonal antibody (80553-2-RR, Clone:242665D1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 90% MeOH . This data was developed using the same antibody clone with 80553-2-PBS in a different storage buffer formulation.

Phospho-TSC2 (Ser1387) Recombinant monoclonal antibody, PBS Only

Phospho-TSC2 (Ser1387) Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 80553-2-PBS

产品说明书

CloneNo. 242665D1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Indirect ELISA

别名

Phospho-Tuberin (Ser1387), Phospho-Tuberin/TSC2 (Ser1387), Phos-TSC2 (Ser1387), Phos-Tuberin (Ser1387), P-TSC2 (Ser1387)

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TSC2 Ready-To-Use IHC Kit. TSC2 primary antibody included.

产品信息

80553-2-PBS targets Phospho-TSC2 (Ser1387) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tuberous sclerosis 2
别名 Phospho-Tuberin (Ser1387), Phospho-Tuberin/TSC2 (Ser1387), Phos-TSC2 (Ser1387), Phos-Tuberin (Ser1387), P-TSC2 (Ser1387)
计算分子量 1807 aa, 201 kDa
观测分子量200 kDa
GenBank蛋白编号BC150300
基因名称 TSC2
Gene ID (NCBI) 7249
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP49815
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TSC2, also named as Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. AMPK can phosphorylate and activate the Ser1387 site of TSC2.(PMID: 14651849, 12172553, PMID: 23244622)

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