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验证数据展示

  • WB analysis using 82382-2-RR

    WB analysis using 82382-2-RR

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82382-2-RR (Phospho-TBK1 (Ser172) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • FC experiment of HeLa using 82382-2-RR

    FC experiment of HeLa using 82382-2-RR

    1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.06 ug Phospho-TBK1 (Ser172) Recombinant antibody (82382-2-RR, Clone:241787G12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-TBK1 (Ser172) Recombinant monoclonal antibody

Phospho-TBK1 (Ser172) Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 82382-2-RR

产品说明书

CloneNo. 241787G12
引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, FC (Intra), ELISA

别名

p TBK1 (Ser172), Phospho TBK1 (Ser172), Phospho-NAK (Ser172), Phospho-TBK1, Phospho-TBK1-Ser172

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCalyculin A treated HeLa cells
Positive FC (Intra) detected inCalyculin A treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy® TBK1 Ready-To-Use IHC Kit. TBK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

82382-2-RR targets Phospho-TBK1 (Ser172) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TANK-binding kinase 1
别名 p TBK1 (Ser172), Phospho TBK1 (Ser172), Phospho-NAK (Ser172), Phospho-TBK1, Phospho-TBK1-Ser172
观测分子量84 kDa
GenBank蛋白编号BC034950
基因名称 TBK1
Gene ID (NCBI) 29110
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UHD2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The serine/threonine protein kinase TBK1, discovered two decades ago as a member of the IκB kinase (IKK) protein kinase family, plays crucial roles in regulating innate immune response by promoting type I interferon (IFN) and modulating NF-κB signaling. The phosphorylation of Ser172 residue on TBK1 is critical and is commonly used as an indicator for its activity. (PMID: 39279883)

实验方案

Product Specific Protocols
WB protocol for Phospho-TBK1 (Ser172) antibody 82382-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Clin Transl Med

Elevation of ISG15 promotes diabetic kidney disease by modulating renal tubular epithelial cell pyroptosis

Authors - Lingzhi Huang
View Article
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