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验证数据展示

  • WB analysis using CL750-81785

    WB analysis using CL750-81785

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-81785 (Phospho-TAK1 (Thr187) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

CoraLite® Plus 750-conjugated Phospho-TAK1 (Thr187) Recombinant antibody

Phospho-TAK1 (Thr187) Uni-rAbTM Recombinant Antibody for WB
Cat No. CL750-81785

产品说明书

CloneNo. 2O6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB

别名

p TAK1, p TAK1 (Thr187), p TAK1 Thr187, Phospho TAK1, Phospho TAK1 (Thr187)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 750
规格: 

-/ -

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经过测试的应用

Positive WB detected inCalyculin A treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy MAP3K7/TAK1 Ready-To-Use IHC Kit. MAP3K7/TAK1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81785-1-PBS

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-81785 targets Phospho-TAK1 (Thr187) in WB applications and shows reactivity with human samples.

经测试应用 WB Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitogen-activated protein kinase kinase kinase 7
别名 p TAK1, p TAK1 (Thr187), p TAK1 Thr187, Phospho TAK1, Phospho TAK1 (Thr187)
计算分子量 579 aa, 64 kDa
观测分子量75-85 kDa
GenBank蛋白编号BC017715
基因名称 TAK1
Gene ID (NCBI) 6885
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43318
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named TAK1 and belongs to the MAP kinase kinase kinase subfamily. It plays an important role in the cascades of cellular responses evoked by changes in the environment. It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825). It has 4 isoforms (53-55 kDa; 64-70 kDa and 75-80 kDa)produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 Phospho-TAK1 (Thr187) antibody CL750-81785Download protocol
Standard Protocols
Click here to view our Standard Protocols