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Phospho-STK11/LKB1 (Thr189) Polyclonal antibody

Phospho-STK11/LKB1 (Thr189) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 28965-1-AP

Print datasheet

Synonyms

LKB1, Phospho-STK11 (Thr189), PJS, serine/threonine kinase 11, STK11

验证数据展示

  • WB analysis using 28965-1-AP

    WB analysis using 28965-1-AP

    Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28965-1-AP (Phospho-STK11/LKB1 (Thr189) antibody) at dilution of 1:10000 incubated at room temperature for 1 hours.

  • WB analysis using 28965-1-AP

    WB analysis using 28965-1-AP

    Non-treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28965-1-AP (Phospho-STK11/LKB1 (Thr189) antibody) at dilution of 1:1000 incubated at 4℃ overnight.

经过测试的应用

Positive WB detected inCalyculin A treated PC-3 cells, UV treated NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy STK11 Ready-To-Use IHC Kit. STK11 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28965-1-AP targets Phospho-STK11/LKB1 (Thr189) in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, mouse
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serine/threonine kinase 11
Synonyms LKB1, Phospho-STK11 (Thr189), PJS, serine/threonine kinase 11, STK11
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC007981
Gene Symbol STK11
Gene ID (NCBI) 6794
RRIDAB_2918225
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15831
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Liver kinase 1 (LKB1 also known as STK11) is a tumor suppressor gene that is inactivated by bi-allelic mutation in non-small cell lung cancer (NSCLC), malignant melanoma and cervical cancer. LKB1 protein has a central kinase domain, two N-terminal nuclear leading sequences, and a C-terminal regulatory motif. The protein also contains a CAAX motif at C-terminal end and can be farnesylated. The formation of an LKB1/Strad/MO25 complex is essential for LKB1's kinase activity, and there are at least 13 known substrates of LKB1, which includes AMPKs, BRSKs, and MARKs. LKB1 is the major kinase that phosphorylates the AMPK activation loop in conditions of energy stress. (PMID: 25679014, PMID: 19629071)

实验方案

Product Specific Protocols
WB protocol for Phospho-STK11/LKB1 (Thr189) antibody 28965-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cells

Psoralen Suppresses Lipid Deposition by Alleviating Insulin Resistance and Promoting Autophagy in Oleate-Induced L02 Cells.

Authors - Yuhao Wang
View Article