验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive FC (Intra) detected in | Calyculin A treated HEK-293T cells |
| Positive ChIP-qPCR detected in | IL-6 (100ng/mL,30min) HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| CHIP-QPCR | CHIP-QPCR : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
80199-2-RR targets Phospho-STAT3 (Ser727) in WB, IHC, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human samples.
| 经测试应用 | WB, FC (Intra), ELISA, ChIP-qPCR Application Description |
| 文献引用应用 | WB, IHC, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | signal transducer and activator of transcription 3 (acute-phase response factor) |
| 别名 | p STAT3, p STAT3 (Ser727), p STAT3 Ser727, Phospho STAT3, Phospho STAT3 (Ser727) |
| 计算分子量 | 770 aa, 88 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC000627 |
| 基因名称 | STAT3 |
| Gene ID (NCBI) | 6774 |
| RRID | AB_3670470 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P40763 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-STAT3 (Ser727) antibody 80199-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Mol Biol Lett The promoting roles of GLP1R and GIPR in stemness maintenance and multiple lineage-specific differentiation of PDLSCs. | ||
Cell Death Discov MFAP2 secreted by TGF-β1-induced cancer-associated fibroblast cells promotes gastric cancer peritoneal metastasis through Src-STAT3-PTK7 axis. | ||
Int J Biol Macromol Matrix metallopeptidase 2-responsive curcumin-loaded nanoparticles-induced signal transducer and activator of transcription 3 inhibition suppresses glioblastoma multiforme growth via enhancing nuclear factor erythroid 2-related factor 2 activity | ||
Food Funct Sulforaphane attenuates DSS-induced ulcerative colitis via the Nrf2/STAT3 signaling pathway and gut microbiota modulation. | ||
Bioresour Bioprocess Mechanism of arbutin in metabolic dysfunction-associated fatty liver disease based on multi-omics research.
| ||
Front Endocrinol (Lausanne) Cangfu Daotan decoction treats PCOS-IR through the IL6/JAK2/STAT3/FOXO4 signaling pathway. |
