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验证数据展示

  • ChIP experiment of HaCaT using 80427-2-RR (same clone as 80427-2-PBS)

    ChIP experiment of HaCaT using 80427-2-RR (same clone as 80427-2-PBS)

    Chromatin was prepared from HaCaT cells treated with TGF-β1 (7 ng/ml) for 1 h. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of Phospho-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) (80427-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 80427-2-PBS in a different storage buffer formulation.

  • WB analysis using 80427-2-RR (same clone as 80427-2-PBS)

    WB analysis using 80427-2-RR (same clone as 80427-2-PBS)

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80427-2-RR (Phospho-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80427-2-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 80427-2-RR (same clone as 80427-2-PBS)

    IF Staining of HEK-293 using 80427-2-RR (same clone as 80427-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed TGF beta 1 treated and untreated HEK-293 cells using Phospho-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) antibody (80427-2-RR, Clone: 240826D11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80427-2-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 80427-2-RR (same clone as 80427-2-PBS)

    FC experiment of HEK-293 using 80427-2-RR (same clone as 80427-2-PBS)

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A were intracellularly stained with 0.13 ug Phospho-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) Recombinant antibody (80427-2-RR, Clone:240826D11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80427-2-PBS in a different storage buffer formulation.

    {{ptg:ProductFeaturesInfo}}

Phospho-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) Recombinant monoclonal antibody, PBS Only

Phospho-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA, ChIP-qPCR
Cat No. 80427-2-PBS

产品说明书

CloneNo. 240826D11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA, ChIP-qPCR

别名

P SMAD2, p SMAD2 (Ser465/467), P SMAD2 (Ser465/467)/SMAD3 (Ser423/425), P SMAD2 Ser465/467 /SMAD3 Ser423/425, p SMAD2/SMAD3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SMAD2 Ready-To-Use IHC Kit. SMAD2 primary antibody included.

产品信息

80427-2-PBS targets Phospho-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) in WB, IF/ICC, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA, ChIP-qPCR Application Description
经测试反应性 human
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SMAD family member 2
别名 P SMAD2, p SMAD2 (Ser465/467), P SMAD2 (Ser465/467)/SMAD3 (Ser423/425), P SMAD2 Ser465/467 /SMAD3 Ser423/425, p SMAD2/SMAD3
计算分子量 467 aa, 52 kDa
观测分子量60 kDa
GenBank蛋白编号BC014840
基因名称 SMAD2
Gene ID (NCBI) 4087
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ15796
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMAD2, also named as MADH2 and MADR2, belongs to the dwarfin/SMAD family, contains 1 MH1 (MAD homology 1) domain and 1 MH2 (MAD homology 2) domain. SMAD2 is a receptor-regulated SMAD(R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. This protein may act as a tumor suppressor in colorectal carcinoma. It is phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, It is phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases, and then able to interact with SMURF2, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, it is phosphorylated on Ser-240 by CaMK2. It is phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. In response to TGF-beta, it is ubiquitinated by NEDD4L, which promotes its degradation. In response to TGF-beta signaling, it is acetylated on Lys-19 by coactivators, which increases transcriptional activity. The molecular weight of unphosphorylated forms of Smad2 is 52 kDa and phosphorylated forms of Smad2 is 58 kDa. (PMID: 9006934)