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验证数据展示

  • WB analysis of MCF-7 using 87071-2-RR (same clone as 87071-2-PBS)

    WB analysis of MCF-7 using 87071-2-RR (same clone as 87071-2-PBS)

    Non-treated MCF-7 cells and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 87071-2-RR (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87071-2-PBS in a different storage buffer formulation.

  • SEC-HPLC of 87071-2-RR

    SEC-HPLC of 87071-2-RR

    The purity of Human Phospho-S6 Ribosomal protein (Ser235/236) Recombinant monoclonal antibody (Cat. No. 87071-2-RR) was greater than 95% as determined by SEC-HPLC.

  • IF Staining of human pancreas using 87071-2-RR (same clone as 87071-2-PBS)

    IF Staining of human pancreas using 87071-2-RR (same clone as 87071-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded λ phosphatase treated human pancreas tissue using Phospho-S6 Ribosomal protein (Ser235/236) antibody (87071-2-RR, Clone: 252281E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87071-2-PBS in a different storage buffer formulation.

  • IF Staining of human pancreas using 87071-2-RR (same clone as 87071-2-PBS)

    IF Staining of human pancreas using 87071-2-RR (same clone as 87071-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human pancreas tissue using Phospho-S6 Ribosomal protein (Ser235/236) antibody (87071-2-RR, Clone: 252281E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87071-2-PBS in a different storage buffer formulation.

Phospho-S6 Ribosomal protein (Ser235/236) Recombinant monoclonal antibody, PBS Only

Phospho-S6 Ribosomal protein (Ser235/236) Uni-rAb® Recombinant Antibody for WB, IF-P, Indirect ELISA
Cat No. 87071-2-PBS

产品说明书

CloneNo. 252281E7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF-P, Indirect ELISA

别名

RPS6, S6, 40S ribosomal protein S6, OK/SW-cl.2, Phosphoprotein NP33

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RPS6 Ready-To-Use IHC Kit. RPS6 primary antibody included.

产品信息

87071-2-PBS targets Phospho-S6 Ribosomal protein (Ser235/236) in WB, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein S6
别名 RPS6, S6, 40S ribosomal protein S6, OK/SW-cl.2, Phosphoprotein NP33
计算分子量 29 kDa
观测分子量32 kDa
GenBank蛋白编号BC000524
基因名称 RPS6
Gene ID (NCBI) 6194
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP62753
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Mitogens also stimulate p90 ribosomal S6 kinases (RSK1-4), which can phosphorylate the same Ser235/236 residues independently of mTOR, providing a parallel pathway that links MAPK activation to translational control. (PMID: 17360704)

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