×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis of MCF-7 using 87071-2-RR

    WB analysis of MCF-7 using 87071-2-RR

    Non-treated MCF-7 cells and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 87071-2-RR (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • SEC-HPLC of 87071-2-RR

    SEC-HPLC of 87071-2-RR

    The purity of Human Phospho-S6 Ribosomal protein (Ser235/236) Recombinant monoclonal antibody (Cat. No. 87071-2-RR) was greater than 95% as determined by SEC-HPLC.

  • IF Staining of human pancreas using 87071-2-RR

    IF Staining of human pancreas using 87071-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded λ phosphatase treated human pancreas tissue using Phospho-S6 Ribosomal protein (Ser235/236) antibody (87071-2-RR, Clone: 252281E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human pancreas using 87071-2-RR

    IF Staining of human pancreas using 87071-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human pancreas tissue using Phospho-S6 Ribosomal protein (Ser235/236) antibody (87071-2-RR, Clone: 252281E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Phospho-S6 Ribosomal protein (Ser235/236) Recombinant monoclonal antibody

Phospho-S6 Ribosomal protein (Ser235/236) Uni-rAb® Recombinant Antibody for WB, IF-P, ELISA
Cat No. 87071-2-RR

产品说明书

CloneNo. 252281E7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF-P, ELISA

别名

RPS6, S6, 40S ribosomal protein S6, OK/SW-cl.2, Phosphoprotein NP33

缓冲液配方:  PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inIGF-1 treated MCF-7 cells
Positive IF-P detected inhuman pancreas tissue
Planning an IHC experiment? We recommend our IHCeasy RPS6 Ready-To-Use IHC Kit. RPS6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87071-2-RR targets Phospho-S6 Ribosomal protein (Ser235/236) in WB, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein S6
别名 RPS6, S6, 40S ribosomal protein S6, OK/SW-cl.2, Phosphoprotein NP33
计算分子量 29 kDa
观测分子量32 kDa
GenBank蛋白编号BC000524
基因名称 RPS6
Gene ID (NCBI) 6194
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP62753
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Mitogens also stimulate p90 ribosomal S6 kinases (RSK1-4), which can phosphorylate the same Ser235/236 residues independently of mTOR, providing a parallel pathway that links MAPK activation to translational control. (PMID: 17360704)

实验方案

Product Specific Protocols
IF protocol for Phospho-S6 Ribosomal protein (Ser235/236) antibody 87071-2-RRDownload protocol
WB protocol for Phospho-S6 Ribosomal protein (Ser235/236) antibody 87071-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...