验证数据展示
经过测试的应用
| Positive WB detected in | Calyculin A treated HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87211-3-RR targets Phospho-RB1 (Ser608) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | retinoblastoma 1 |
| 别名 | Phospho-RB, Phospho-Rb (Ser608), Phospho-RB1, pRb, p-RB |
| 计算分子量 | 928 aa, 106 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | BC039060 |
| 基因名称 | RB1 |
| Gene ID (NCBI) | 5925 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P06400 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. Ser608 is located within the "spacer" region of RB1, which connects the A and B pocket domains. This region is critical for RB1's interaction with various cellular proteins.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-RB1 (Ser608) antibody 87211-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
