验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
88430-1-RR targets Phospho-RAF1 (Ser259) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | v-raf-1 murine leukemia viral oncogene homolog 1 |
| 别名 | c-Raf (Ser259), p-c-Raf, p-c-Raf (Ser259), Phospho-c-Raf, Phospho-c-Raf (Ser259) |
| 计算分子量 | 648 aa, 73 kDa |
| 观测分子量 | 70-75 kDa |
| GenBank蛋白编号 | BC018119 |
| 基因名称 | RAF1 |
| Gene ID (NCBI) | 5894 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P04049 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Phospho-RAF1 (Ser259) is a key inhibitory modification of CRAF within the RAS-MEK-ERK cascade. Phosphorylated mainly by PKA and Akt, this residue recruits 14-3-3 proteins to lock RAF1 in an autoinhibited cytoplasmic conformation, blocking its membrane recruitment and binding to GTP-Ras. Dephosphorylation by PP2A relieves suppression to trigger ERK-driven proliferation. Elevated p-RAF1 (S259) dampens oncogenic MAPK signaling in tumors, while loss of this phosphorylation causes sustained pathway hyperactivation linked to Noonan syndrome and multiple malignancies. (PMID: 11971957, 11756411)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-RAF1 (Ser259) antibody 88430-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
