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验证数据展示

  • WB analysis using 29564-1-AP (same clone as 29564-1-PBS)

    WB analysis using 29564-1-AP (same clone as 29564-1-PBS)

    Non-treated and PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 29564-1-AP (Phospho-PKC Delta (Thr507) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with PKC Delta antibody (19132-1-AP) subsequently. This data was developed using the same antibody clone with 29564-1-PBS in a different storage buffer formulation.

Phospho-PKC Delta (Thr507) Polyclonal antibody, PBS Only

Phospho-PKC Delta (Thr507) Polyclonal Antibody for WB, Indirect ELISA
Cat No. 29564-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

PKC delta, PRKCD, PKCD, nPKC-delta, MAY1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PRKCD Ready-To-Use IHC Kit. PRKCD primary antibody included.

产品信息

29564-1-PBS targets Phospho-PKC Delta (Thr507) in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase C, delta
别名 PKC delta, PRKCD, PKCD, nPKC-delta, MAY1
计算分子量 78 kDa
观测分子量70 kDa
GenBank蛋白编号BC043350
基因名称 PKC Delta
Gene ID (NCBI) 5580
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ05655
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PKC Delta(PRKCD) is a member of the PKC family, a phospholipid-dependent family of serine/threonine kinases, and is present in human neutrophils. Novel PKCs (PRKCD, PRKCE, PRKCH and PRKCQ) are calcium-insensitive, but activated by diacylglycerol (DAG) and phosphatidylserine. Three specific sites; Thr-507 (activation loop of the kinase domain), Ser-645 (turn motif) and Ser-664 (hydrophobic region), need to be phosphorylated for its full activation. This antibody is equivalent to Thr505 in mice.