验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, PMA treated HL-60 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29123-1-AP targets Phospho-PKC Alpha (Thr638) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, chicken |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein kinase C, alpha |
| 别名 | p PKC Alpha, p PKC Alpha (Thr638), p PKC Alpha Thr638, Phospho PKC Alpha, Phospho PKC Alpha (Thr638) |
| 计算分子量 | 77 kDa |
| 观测分子量 | 77 kDa |
| GenBank蛋白编号 | AK055431 |
| 基因名称 | PKC Alpha |
| Gene ID (NCBI) | 5578 |
| RRID | AB_2918239 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P17252 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PKCs are a family of serine/threonine kinases involved in various processes in cells including proliferation, differentiation, cell survival, and apoptosis. PKC family is composed of three different subgroups: conventional (cPKC), the novel (nPKC) and atypical (aPKC). PKCα, β1, β2, and γ belong to cPKC, PKCδ, ε, η and θ are nPKC, whereas aPKC comprises of PKCζ and λ/ι. All PKCs consist of the N-terminal regulatory region and C-terminal catalytic region (kinase domain). PKCs are physiologically activated by various extracellular signals transduced by hormones, growth factors, cytokines or antigens. The presence of activated PKCs on internal membranes leads to the phosphorylation of various interacting proteins. (PMID: 32466765, PMID: 12417016)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-PKC Alpha (Thr638) antibody 29123-1-AP | Download protocol |
| WB protocol for Phospho-PKC Alpha (Thr638) antibody 29123-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Med Cholinergic signaling via muscarinic M1 receptor confers resistance to docetaxel in prostate cancer | ||
Mol Psychiatry PIAS1-mediated GSK3β SUMOylation exacerbates tauopathy and cognitive deficits in Alzheimer's disease models. | ||
Neurotherapeutics Regulatory T cells attenuate astrogliosis via IL-10/STAT3/PKC/vimentin signaling and promote neurological recovery after spinal cord injury. | ||
Biochem Pharmacol Sodium butyrate upregulates URAT1-mediated urate reabsorption in HK2 cells by activating FFAR2/p38 MAPK/ELK1/ARRB2 axis and relieving NF-κB-HNF1β competitive binding. | ||
Biochem Pharmacol IGFBP5 suppresses colorectal cancer stemness and carcinogenesis via binding to GPR182 and inhibiting PKCα/AKT/P70S6K axis. | ||
Eur J Pharmacol Salicylic acid inhibits Mas-related G protein-coupled receptor X2-mediated mast cell 2 activation and mitigates cutaneous pseudo-allergic reactions. |
