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验证数据展示

  • WB analysis using 82534-1-RR (same clone as 82534-1-PBS)

    WB analysis using 82534-1-RR (same clone as 82534-1-PBS)

    Non-treated HEK-293 cells and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 82534-1-RR (Phospho-PERK/EIF2AK3 (Thr982) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with beta actin antibody (66009-1-Ig) as loading control. This data was developed using the same antibody clone with 82534-1-PBS in a different storage buffer formulation.

  • WB analysis using 82534-1-RR (same clone as 82534-1-PBS)

    WB analysis using 82534-1-RR (same clone as 82534-1-PBS)

    Non-treated NIH/3T3 cells and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82534-1-RR (Phospho-PERK/EIF2AK3 (Thr982) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82534-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 82534-1-RR (same clone as 82534-1-PBS)

    FC experiment of HEK-293 using 82534-1-RR (same clone as 82534-1-PBS)

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug Phospho-PERK/EIF2AK3 (Thr982) Recombinant antibody (82534-1-RR, Clone:4E16) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 82534-1-PBS in a different storage buffer formulation.

Phospho-PERK/EIF2AK3 (Thr982) Recombinant antibody, PBS Only

Phospho-PERK/EIF2AK3 (Thr982) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 82534-1-PBS

产品说明书

CloneNo. 4E16

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Indirect ELISA

别名

p PERK/EIF2AK3, p PERK/EIF2AK3 (Thr982), p PERK/EIF2AK3 Thr982, PERK/EIF2AK3 (Thr982), Phospho PERK/EIF2AK3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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This antibody is equivalent to Thr980 in mice.

产品信息

82534-1-PBS targets Phospho-PERK/EIF2AK3 (Thr982) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 eukaryotic translation initiation factor 2-alpha kinase 3
别名 p PERK/EIF2AK3, p PERK/EIF2AK3 (Thr982), p PERK/EIF2AK3 Thr982, PERK/EIF2AK3 (Thr982), Phospho PERK/EIF2AK3
计算分子量 1116 aa, 125 kDa
观测分子量180 kDa
GenBank蛋白编号BC126354
基因名称 PERK
Gene ID (NCBI) 9451
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NZJ5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)