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验证数据展示

  • WB analysis using 80688-2-RR

    WB analysis using 80688-2-RR

    Non-treated HeLa cells, phosphatase inhibitor and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 80688-2-RR (Phospho-PDH E1 Alpha (Ser293) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

Phospho-PDH E1 Alpha (Ser293) Recombinant antibody

Phospho-PDH E1 Alpha (Ser293) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 80688-2-RR

产品说明书

CloneNo. 250640D9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

P PDH E1 Alpha (Ser293), Phos PDH E1 Alpha (Ser293), Phos-PDH E1 Alpha (Ser293), Phospho-PDHA1 (Ser293), Phospho-Pyruvate Dehydrogenase α1 (Ser293)

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, λ phosphatase treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy PDHA1 Ready-To-Use IHC Kit. PDHA1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80688-2-RR targets Phospho-PDH E1 Alpha (Ser293) in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pyruvate dehydrogenase (lipoamide) alpha 1
别名 P PDH E1 Alpha (Ser293), Phos PDH E1 Alpha (Ser293), Phos-PDH E1 Alpha (Ser293), Phospho-PDHA1 (Ser293), Phospho-Pyruvate Dehydrogenase α1 (Ser293)
计算分子量 43 kDa
观测分子量40 kDa
GenBank蛋白编号BC002406
基因名称 PDHA1
Gene ID (NCBI) 5160
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08559
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDH E1 Alpha (PDHA1), as the major component of PDH, can be phosphorylated and inactivated by PDHK1. PDHA1 contains three serine residues (Ser232, Ser293, or Ser300) that can be reversibly phosphorylated by a dedicated family of four inhibitory pyruvate dehydrogenase kinases (PDHK1-4) and two reactivating phosphatases (PDP1, 2). Hypoxia induces the expression of PDHK1 and PDHK3 and hyperphosphorylates PDHA1. Phosphorylated PDHA1 inactivates the whole pyruvate dehydrogenase complex (PDC), reduces pyruvate entering into the tricarboxylic acid (TCA) cycle for oxidative phosphorylation, enhances the Warburg effect and promotes tumorigenesis. (PMID: 30993888, PMID: 34749809)

实验方案

Product Specific Protocols
WB protocol for Phospho-PDH E1 Alpha (Ser293) antibody 80688-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols